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Literature summary extracted from

  • Hall, A.M.; Wiczer, B.M.; Herrmann, T.; Stremmel, W.; Bernlohr, D.A.
    Enzymatic properties of purified murine fatty acid transport protein 4 and analysis of acyl-CoA synthetase activities in tissues from FATP4 null mice (2005), J. Biol. Chem., 280, 11948-11954.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.3 expression in COS1 cells Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.3 Triacsin C no effect on FATP4 acyl-CoA synthetase activity towards n-tetracosanoic acid, inhibits activity with palmitate with IC50 of 0.03 mM Mus musculus
6.2.1.3 troglitazone no effect on FATP4 acyl-CoA synthetase activity towards n-tetracosanoic acid up to 0.05 mM, inhibits activity with palmitate with IC50 of 0.02 mM Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.3 0.0048
-
 n-tetracosanoic acid
-
 Mus musculus
6.2.1.3 0.013
-
 palmitate
-
 Mus musculus
6.2.1.3 0.047
-
 CoA
-
 Mus musculus
6.2.1.3 1.4
-
 ATP
-
 Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.3 additional information Mus musculus in vivo the loss of FATP4-mediated very long chain fatty acid uptake and/or esterification may underlie the null phenotype ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.3 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.3
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.3 brain
-
 Mus musculus
-
6.2.1.3 intestine
-
 Mus musculus
-
6.2.1.3 liver
-
 Mus musculus
-
6.2.1.3 lung
-
 Mus musculus
-
6.2.1.3 skin
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.3 0.723
-
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.3 ATP + n-tetracosanoic acid + CoA
-
 Mus musculus AMP + diphosphate + n-tetracosanoyl-CoA
-
 ?
6.2.1.3 ATP + palmitate + CoA
-
 Mus musculus AMP + diphosphate + palmitoyl-CoA
-
 ?
6.2.1.3 additional information in vivo the loss of FATP4-mediated very long chain fatty acid uptake and/or esterification may underlie the null phenotype Mus musculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.3 FATP4 bifunctional enzyme, exhibiting both fatty acid transport and acyl-CoA synthetase activities Mus musculus
6.2.1.3 fatty acid transport protein bifunctional enzyme, exhibiting both fatty acid transport and acyl-CoA synthetase activities Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.3 7.5
-
 substrate: palmitate Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.2.1.3 6 9 about 60% of maximal activity at pH 6.0 and pH 9.0 Mus musculus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.2.1.3 0.02
-
 no effect on FATP4 acyl-CoA synthetase activity towards n-tetracosanoic acid up to 0.05 mM, inhibits activity with palmitate with IC50 of 0.02 mM Mus musculus troglitazone
6.2.1.3 0.03
-
 no effect on FATP4 acyl-CoA synthetase activity towards n-tetracosanoic acid, inhibits activity with palmitate with IC50 of 0.03 mM Mus musculus Triacsin C