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Literature summary extracted from

  • Koropatkin, N.M.; Cleland, W.W.; Holden, H.M.
    Kinetic and structural analysis of alpha-D-glucose-1-phosphate cytidylyltransferase from Salmonella typhi (2005), J. Biol. Chem., 280, 10774-10780.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.33 hanging-drop vapour-diffusion method.The structure of the enzyme with bound MgCTP reveals that the binding pocket for the nucleotide is contained within one subunit rather than shared between two. Key side chains involved in nucleotide binding include Thr14, Arg15, Lys25, and Arg111 Salmonella enterica subsp. enterica serovar Typhi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.33 0.011
-
 CDP-glucose
-
 Salmonella enterica subsp. enterica serovar Typhi
2.7.7.33 0.052
-
 alpha-D-glucose 1-phosphate
-
 Salmonella enterica subsp. enterica serovar Typhi
2.7.7.33 0.067
-
 diphosphate
-
 Salmonella enterica subsp. enterica serovar Typhi
2.7.7.33 0.148
-
 CTP
-
 Salmonella enterica subsp. enterica serovar Typhi
2.7.7.33 2.535
-
 UDP-glucose
-
 Salmonella enterica subsp. enterica serovar Typhi

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.33 Salmonella enterica subsp. enterica serovar Typhi
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.33 CTP + alpha-D-glucose 1-phosphate the structure of the enzyme with bound MgCTP reveals that the binding pocket for the nucleotide is contained within one subunit rather than shared between two. Key side chains involved in nucleotide binding include Thr14, Arg15, Lys25, and Arg111 Salmonella enterica subsp. enterica serovar Typhi diphosphate + CDP-glucose
-
 r
2.7.7.33 UTP + alpha-D-glucose 1-phosphate
-
 Salmonella enterica subsp. enterica serovar Typhi diphosphate + UDP-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.33 alpha-D-glucose-1-phosphate cytidylyltransferase
-
 Salmonella enterica subsp. enterica serovar Typhi