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Literature summary extracted from

  • Gomez-Merino, F.C.; Brearley, C.A.; Ornatowska, M.; Abdel-Haliem, M.E.; Zanor, M.I.; Mueller-Roeber, B.
    AtDGK2, a novel diacylglycerol kinase from Arabidopsis thaliana, phosphorylates 1-stearoyl-2-arachidonoyl-sn-glycerol and 1,2-dioleoyl-sn-glycerol and exhibits cold-inducible gene expression (2004), J. Biol. Chem., 279, 8230-8241.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.107 additional information the isozyme DGK2 is induced by exposure to low temperatures, e.g. 4°C Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.107 AtDGK2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as NusA/His6-tagged isozyme DGK2 in Escherichia coli strain BL21(DE3) Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.107 LiCl more than 50% inhibition at above 200 mM Arabidopsis thaliana
2.7.1.107 additional information isozyme DGK2 activity is affected by salts and detergents, no inhibition by Ca2+ Arabidopsis thaliana
2.7.1.107 NaCl more than 50% inhibition at above 200 mM Arabidopsis thaliana
2.7.1.107 R59022 specific inhibitor Arabidopsis thaliana
2.7.1.107 sodium deoxycholate at 5 mM Arabidopsis thaliana
2.7.1.107 Triton X-100 strong inhibition at up to 2% Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.107 0.125
-
 1,2-dioleoyl-sn-glycerol pH 7.5, 25°C Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.107 Mg2+ dependent on Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.107 79400
-
 x * 79400, about, sequence calculation, x * 140000, about, recombinant NusA/His6-tagged isozyme DGK2, SDS-PAGE Arabidopsis thaliana
2.7.1.107 140000
-
 x * 79400, about, sequence calculation, x * 140000, about, recombinant NusA/His6-tagged isozyme DGK2, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.107 ATP + 1,2-diacylglycerol Arabidopsis thaliana
-
 ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 additional information Arabidopsis thaliana the isozyme DGK2 is involved in cold signal transduction ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.107 Arabidopsis thaliana Q9FFN7 2 isozymes DGK1 and DGK2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.107 CHAPS is the most effective detergent for isozyme DGK2 solubilization, NusA/His6-tagged fusion isozyme DGK2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.107 flower
-
 Arabidopsis thaliana
-
2.7.1.107 leaf especially cauline Arabidopsis thaliana
-
2.7.1.107 additional information isozyme DGK2 expression in various tissues, expression pattern Arabidopsis thaliana
-
2.7.1.107 root
-
 Arabidopsis thaliana
-
2.7.1.107 silique
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.107 additional information
-
-
 Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.107 ATP + 1,2-diacylglycerol
-
 Arabidopsis thaliana ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 ATP + 1,2-diacylglycerol
-
 Arabidopsis thaliana ADP + 1,2-diacyl-sn-glycerol 3-phosphate i.e. phosphatidic acid ?
2.7.1.107 ATP + 1,2-dioleoyl-sn-glycerol
-
 Arabidopsis thaliana ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol
-
 Arabidopsis thaliana ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 additional information the isozyme DGK2 is involved in cold signal transduction Arabidopsis thaliana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.107 ? x * 79400, about, sequence calculation, x * 140000, about, recombinant NusA/His6-tagged isozyme DGK2, SDS-PAGE Arabidopsis thaliana
2.7.1.107 More structural organization of isozymes containing a 1,2-diacylglycerol/phorbolester-binding domain 1, a zinc-finger dependent C1 domain, and a 1,2-diacylglycerol accessory domain, overview Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.7.1.107 DGK2
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.107 25
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.107 7.2
-
-
 Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.107 6.5 7.8 rapid decrease below pH 6.5 and above pH 7.8 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.107 ATP
-
 Arabidopsis thaliana