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Literature summary extracted from

  • Zawadzki, K.M.; Taylor, S.S.
    cAMP-dependent protein kinase regulatory subunit type IIbeta: active site mutations define an isoform-specific network for allosteric signaling by cAMP (2004), J. Biol. Chem., 279, 7029-7036.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.11 cAMP each monomer of the tetrameric enzyme contains 2 bindig sites A and B for cAMP, binding involves Arg230 of domain A and Arg359 in domain B of regulatory subunit RIIbeta, cAMP binding releases the catalytic subunits Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.11 expression of His-tagged catalytic subunit C, and His-tagged wild-type and mutant regulatory subunits RIIbeta Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.11 additional information construction of a deletion mutant of regulatory subunit RIIbeta lacking the complete domain B Rattus norvegicus
2.7.11.11 R230K site-directed mutagenesis, mutation of an A domain residue of regulatory subunit RIIbeta, the mutant possesses one high-affinity cAMP binding site and one low active binding site resulting in reduced overall cAMP binding Rattus norvegicus
2.7.11.11 R359K site-directed mutagenesis, mutation of a B domain residue of regulatory subunit RIIbeta, the mutant possesses one high-affinity cAMP binding site and one low active binding site resulting in reduced overall cAMP binding Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.11 additional information
-
additional information kinetics for cAMP binding to wild-type and mutant regulatory subunits RIIbeta, overview Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.11 Mg2+
-
Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.11 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.11 recombinant His-tagged catalytic subunit C, and His-tagged wild-type and mutant regulatory subunits RIIbeta, by nickel affinity chromatography and dialysis Rattus norvegicus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.7.11.11 reversible unfolding and folding in 8.5 M urea of recombinant His-tagged wild-type and mutant regulatory subunits RIIbeta, overview Rattus norvegicus

Subunits

EC Number Subunits Comment Organism
2.7.11.11 More holoenzyme structure Rattus norvegicus
2.7.11.11 tetramer 2 regulatory subunits in a dimer bound to 2 catalytic subunits, the latter are released during activation by cAMP Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.7.11.11 cAPK
-
Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.11 ATP
-
Rattus norvegicus