Literature summary extracted from
Zawadzki, K.M.; Taylor, S.S.
cAMP-dependent protein kinase regulatory subunit type IIbeta: active site mutations define an isoform-specific network for allosteric signaling by cAMP (2004), J. Biol. Chem., 279, 7029-7036.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
2.7.11.11 |
cAMP |
each monomer of the tetrameric enzyme contains 2 bindig sites A and B for cAMP, binding involves Arg230 of domain A and Arg359 in domain B of regulatory subunit RIIbeta, cAMP binding releases the catalytic subunits |
Rattus norvegicus |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.7.11.11 |
expression of His-tagged catalytic subunit C, and His-tagged wild-type and mutant regulatory subunits RIIbeta |
Rattus norvegicus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.7.11.11 |
additional information |
construction of a deletion mutant of regulatory subunit RIIbeta lacking the complete domain B |
Rattus norvegicus |
2.7.11.11 |
R230K |
site-directed mutagenesis, mutation of an A domain residue of regulatory subunit RIIbeta, the mutant possesses one high-affinity cAMP binding site and one low active binding site resulting in reduced overall cAMP binding |
Rattus norvegicus |
2.7.11.11 |
R359K |
site-directed mutagenesis, mutation of a B domain residue of regulatory subunit RIIbeta, the mutant possesses one high-affinity cAMP binding site and one low active binding site resulting in reduced overall cAMP binding |
Rattus norvegicus |
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
2.7.11.11 |
additional information |
- |
additional information |
kinetics for cAMP binding to wild-type and mutant regulatory subunits RIIbeta, overview |
Rattus norvegicus |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
2.7.11.11 |
Mg2+ |
- |
Rattus norvegicus |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.11.11 |
Rattus norvegicus |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.7.11.11 |
recombinant His-tagged catalytic subunit C, and His-tagged wild-type and mutant regulatory subunits RIIbeta, by nickel affinity chromatography and dialysis |
Rattus norvegicus |
Renatured (Commentary)
EC Number |
Renatured (Comment) |
Organism |
---|
2.7.11.11 |
reversible unfolding and folding in 8.5 M urea of recombinant His-tagged wild-type and mutant regulatory subunits RIIbeta, overview |
Rattus norvegicus |
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.7.11.11 |
More |
holoenzyme structure |
Rattus norvegicus |
2.7.11.11 |
tetramer |
2 regulatory subunits in a dimer bound to 2 catalytic subunits, the latter are released during activation by cAMP |
Rattus norvegicus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.7.11.11 |
cAPK |
- |
Rattus norvegicus |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
2.7.11.11 |
ATP |
- |
Rattus norvegicus |
|