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Literature summary extracted from

  • Noda, M.; Kawahara, Y.; Ichikawa, A.; Matoba, Y.; Matsuo, H.; Lee, D.G.; Kumagai, T.; Sugiyama, M.
    Self-protection mechanism in D-cycloserine-producing Streptomyces lavendulae. Gene cloning, characterization, and kinetics of its alanine racemase and D-alanyl-D-alanine ligase, which are target enzymes of D-cycloserine (2004), J. Biol. Chem., 279, 46143-46152.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.1
-
 Escherichia coli
5.1.1.1
-
 Streptomyces lavendulae
6.3.2.4 expressed in Escherichia coli BL21 Streptomyces lavendulae

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.1.1 D-cycloserine time-dependent inactivation rate of enzyme from Streptomyces lavendulae is slower than for enzyme from Escherichia coli Escherichia coli
5.1.1.1 D-cycloserine time-dependent inactivation rate of enzyme from Streptomyces lavendulae is slower than for enzyme from Escherichia coli. Enzyme from Streptomyces lavendulae is one of its self-resistance determinants Streptomyces lavendulae
5.1.1.1 L-Cycloserine
-
 Escherichia coli
5.1.1.1 additional information not inhibitory: L-cycloserine Streptomyces lavendulae
6.3.2.4 D-alanyl-D-alanine
-
 Streptomyces lavendulae
6.3.2.4 D-cycloserine
-
 Streptomyces lavendulae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.1 0.7
-
 L-alanine pH 8.2, 25°C Streptomyces lavendulae
5.1.1.1 0.7
-
 D-alanine pH 8.2, 25°C Streptomyces lavendulae
6.3.2.4 0.12
-
 ATP
-
 Streptomyces lavendulae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.1.1.1 soluble
-
 Streptomyces lavendulae
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.1.1 39900
-
 2 * 39900, calculated, 2 * 42000, SDs-PAGE Escherichia coli
5.1.1.1 39900
-
 2 * 39900, calculated, 2 * 42000, SDs-PAGE Streptomyces lavendulae
5.1.1.1 42000
-
 2 * 39900, calculated, 2 * 42000, SDs-PAGE Escherichia coli
5.1.1.1 42000
-
 2 * 39900, calculated, 2 * 42000, SDs-PAGE Streptomyces lavendulae
5.1.1.1 80000
-
 gel filtration Escherichia coli
5.1.1.1 80000
-
 gel filtration Streptomyces lavendulae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.4 ATP + D-alanine Streptomyces lavendulae
-
 ADP + phosphate + D-alanyl-D-alanine
-
 ?
6.3.2.4 ATP + D-alanine Streptomyces lavendulae ATCC 25233
-
 ADP + phosphate + D-alanyl-D-alanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.1 Escherichia coli
-
-
-
5.1.1.1 Streptomyces lavendulae Q65YW7
-
-
6.3.2.4 Streptomyces lavendulae
-
 strain ATCC25233
-
6.3.2.4 Streptomyces lavendulae ATCC 25233
-
 strain ATCC25233
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.1 recombinant protein Escherichia coli
5.1.1.1 recombinant protein Streptomyces lavendulae
6.3.2.4
-
 Streptomyces lavendulae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.1 D-alanine
-
 Escherichia coli L-alanine
-
 r
5.1.1.1 D-alanine
-
 Streptomyces lavendulae L-alanine
-
 r
5.1.1.1 L-alanine
-
 Escherichia coli D-alanine
-
 r
5.1.1.1 L-alanine
-
 Streptomyces lavendulae D-alanine
-
 r
6.3.2.4 ATP + D-alanine
-
 Streptomyces lavendulae ADP + phosphate + D-alanyl-D-alanine
-
 ?
6.3.2.4 ATP + D-alanine
-
 Streptomyces lavendulae ATCC 25233 ADP + phosphate + D-alanyl-D-alanine
-
 ?
6.3.2.4 D-cycloserine
-
 Streptomyces lavendulae ?
-
 ?
6.3.2.4 D-cycloserine
-
 Streptomyces lavendulae ATCC 25233 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.1.1 dimer 2 * 39900, calculated, 2 * 42000, SDs-PAGE Escherichia coli
5.1.1.1 dimer 2 * 39900, calculated, 2 * 42000, SDs-PAGE Streptomyces lavendulae

Synonyms

EC Number Synonyms Comment Organism
6.3.2.4 Ddl
-
 Streptomyces lavendulae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.1.1 55
-
 L-alanine pH 8.2, 25°C Streptomyces lavendulae
5.1.1.1 70
-
 D-alanine pH 8.2, 25°C Streptomyces lavendulae
6.3.2.4 1.67
-
 D-alanine
-
 Streptomyces lavendulae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.2.4 6 9.2 higher DDL activity at pH 9.2 than at pH 6.0-7.5 Streptomyces lavendulae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.1.1.1 0.87
-
 D-cycloserine pH 8.2, 25°C Streptomyces lavendulae
5.1.1.1 1.2
-
 D-cycloserine pH 8.2, 25°C Escherichia coli
5.1.1.1 8.2
-
 L-Cycloserine pH 8.2, 25°C Escherichia coli
6.3.2.4 0.06
-
 D-alanyl-D-alanine
-
 Streptomyces lavendulae
6.3.2.4 0.92
-
 D-cycloserine
-
 Streptomyces lavendulae