EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.7.4 | truncated enzyme form | Penicillium chrysogenum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.7.4 | del396-573 | recombinant ATP sulfurylase lacking the C-terminal allosteric domain is monomeric and noncooperative. Mutant enzyme is less heat stable than wild-type enzyme. Wild-type enzyme behaves cooperative at pH 6.5, truncated enzyme form displays normal hyperbolic behavior | Penicillium chrysogenum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.4 | 3'-phosphoadenosine 5'-phosphosulfate | allosteric, binding of the inhibitor to the catalytic site as well as to the allosteric site of the wild type enzyme acts to decrease the degree of cooperativity | Penicillium chrysogenum | |
2.7.7.4 | 5,5'-dithiobis(2-nitrobenzoic acid) | 0.05 mM, rapid decrease in activity of wild-type enzyme (t1/2: 20 s), truncated enzyme del396-573 retains more than 97% of its activity after 30 min | Penicillium chrysogenum | |
2.7.7.4 | adenosine 5'-phosphosulfate | competitive with both substrates | Penicillium chrysogenum | |
2.7.7.4 | diphosphate | noncompetitive with respect to MgATP and sulfate | Penicillium chrysogenum | |
2.7.7.4 | FSO3- | 0.03 mM, 50% inhibition | Penicillium chrysogenum | |
2.7.7.4 | NEM | 0.15 mM, rapid decrease in activity of wild-type enzyme (t1/2: 45 s), truncated enzyme del396-573 retains more than 97% of its activity after 30 min | Penicillium chrysogenum | |
2.7.7.4 | Phenylglyoxal | 3 mM, irreversible inactivation of wild-type enzyme and mutant enzyme del396-573, t1/2: 5 min for both forms | Penicillium chrysogenum | |
2.7.7.4 | thiosulfate | competitive with molybdate and noncompetitive with MgATP | Penicillium chrysogenum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.4 | 0.0004 | - |
adenylylsulfate | pH 8.0, 30°C, wild-type enzyme | Penicillium chrysogenum | |
2.7.7.4 | 0.0005 | - |
adenylylsulfate | pH 8.0, 30°C, truncated mutant enzyme del396-573 | Penicillium chrysogenum | |
2.7.7.4 | 0.0092 | - |
diphosphate | pH 8.0, 30°C, wild-type enzyme | Penicillium chrysogenum | |
2.7.7.4 | 0.025 | - |
diphosphate | pH 8.0, 30°C, truncated mutant enzyme del396-573 | Penicillium chrysogenum | |
2.7.7.4 | 0.027 | - |
ATP | pH 8.0, 30°C, molybdolysis, truncated mutant enzyme del396-573 | Penicillium chrysogenum | |
2.7.7.4 | 0.027 | - |
ATP | pH 8.0, 30°C, molybdolysis, wild-type enzyme | Penicillium chrysogenum | |
2.7.7.4 | 0.076 | - |
MoO42- | pH 8.0, 30°C, wild-type enzyme | Penicillium chrysogenum | |
2.7.7.4 | 0.21 | - |
ATP | pH 8.0, 30°C, synthesis of adenylylsulfate, wild-type enzyme | Penicillium chrysogenum | |
2.7.7.4 | 0.29 | - |
sulfate | pH 8.0, 30°C, wild-type enzyme | Penicillium chrysogenum | |
2.7.7.4 | 0.53 | - |
MoO42- | pH 8.0, 30°C, truncated mutant enzyme del396-573 | Penicillium chrysogenum | |
2.7.7.4 | 2.6 | - |
ATP | pH 8.0, 30°C, synthesis of adenylylsulfate, truncated mutant enzyme del396-573 | Penicillium chrysogenum | |
2.7.7.4 | 3.6 | - |
sulfate | pH 8.0, 30°C, truncated mutant enzyme del396-573 | Penicillium chrysogenum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.4 | Penicillium chrysogenum | Q12650 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.4 | ATP + MoO42- | - |
Penicillium chrysogenum | AMP + adenylylmolybdate | - |
r | |
2.7.7.4 | ATP + sulfate | - |
Penicillium chrysogenum | diphosphate + adenylylsulfate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.7.4 | hexamer | wild-type enzyme | Penicillium chrysogenum |
2.7.7.4 | monomer | 1 * 46000,truncated mutant enzyme del396-573, SDS-PAGE | Penicillium chrysogenum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.4 | 45 | - |
t1/2 for mutant enzyme del396-573: 1.5 min | Penicillium chrysogenum |
2.7.7.4 | 50 | - |
t1/2 for mutant enzyme del396-573: 0.3 min, wild-type enzyme is stable for more than 2 h | Penicillium chrysogenum |
2.7.7.4 | 62 | - |
t1/2 for wild-type enzyme: 1.5 min | Penicillium chrysogenum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.4 | 1.8 | - |
ATP | pH 8.0, 30°C, synthesis of adenylylsulfate, truncated mutant enzyme del396-573 | Penicillium chrysogenum | |
2.7.7.4 | 1.8 | - |
sulfate | pH 8.0, 30°C, truncated mutant enzyme del396-573 | Penicillium chrysogenum | |
2.7.7.4 | 10.8 | - |
ATP | pH 8.0, 30°C, synthesis of adenylylsulfate, wild-type enzyme | Penicillium chrysogenum | |
2.7.7.4 | 10.8 | - |
sulfate | pH 8.0, 30°C, wild-type enzyme | Penicillium chrysogenum | |
2.7.7.4 | 13.7 | - |
ATP | pH 8.0, 30°C, molybdolysis, truncated mutant enzyme del396-573 | Penicillium chrysogenum | |
2.7.7.4 | 13.7 | - |
MoO42- | pH 8.0, 30°C,truncated mutant enzyme del396-573 | Penicillium chrysogenum | |
2.7.7.4 | 24.4 | - |
ATP | pH 8.0, 30°C, molybdolysis, wild-type enzyme | Penicillium chrysogenum | |
2.7.7.4 | 24.4 | - |
MoO42- | pH 8.0, 30°C, wild-type enzyme | Penicillium chrysogenum | |
2.7.7.4 | 46.6 | - |
adenylylsulfate | pH 8.0, 30°C, truncated mutant enzyme del396-573 | Penicillium chrysogenum | |
2.7.7.4 | 46.6 | - |
adenylylsulfate | pH 8.0, 30°C, wild-type enzyme | Penicillium chrysogenum | |
2.7.7.4 | 73.3 | - |
diphosphate | pH 8.0, 30°C, truncated mutant enzyme del396-573 | Penicillium chrysogenum | |
2.7.7.4 | 73.3 | - |
diphosphate | pH 8.0, 30°C, wild-type enzyme | Penicillium chrysogenum |