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Literature summary extracted from

  • Hanna, E.; Ng, K.F.; MacRae, I.J.; Bley, C.J.; Fisher, A.J.; Segel, I.H.
    Kinetic and stability properties of Penicillium chrysogenum ATP sulfurylase missing the C-terminal regulatory domain (2004), J. Biol. Chem., 279, 4415-4424.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.4 truncated enzyme form Penicillium chrysogenum

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.4 del396-573 recombinant ATP sulfurylase lacking the C-terminal allosteric domain is monomeric and noncooperative. Mutant enzyme is less heat stable than wild-type enzyme. Wild-type enzyme behaves cooperative at pH 6.5, truncated enzyme form displays normal hyperbolic behavior Penicillium chrysogenum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.4 3'-phosphoadenosine 5'-phosphosulfate allosteric, binding of the inhibitor to the catalytic site as well as to the allosteric site of the wild type enzyme acts to decrease the degree of cooperativity Penicillium chrysogenum
2.7.7.4 5,5'-dithiobis(2-nitrobenzoic acid) 0.05 mM, rapid decrease in activity of wild-type enzyme (t1/2: 20 s), truncated enzyme del396-573 retains more than 97% of its activity after 30 min Penicillium chrysogenum
2.7.7.4 adenosine 5'-phosphosulfate competitive with both substrates Penicillium chrysogenum
2.7.7.4 diphosphate noncompetitive with respect to MgATP and sulfate Penicillium chrysogenum
2.7.7.4 FSO3- 0.03 mM, 50% inhibition Penicillium chrysogenum
2.7.7.4 NEM 0.15 mM, rapid decrease in activity of wild-type enzyme (t1/2: 45 s), truncated enzyme del396-573 retains more than 97% of its activity after 30 min Penicillium chrysogenum
2.7.7.4 Phenylglyoxal 3 mM, irreversible inactivation of wild-type enzyme and mutant enzyme del396-573, t1/2: 5 min for both forms Penicillium chrysogenum
2.7.7.4 thiosulfate competitive with molybdate and noncompetitive with MgATP Penicillium chrysogenum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.4 0.0004
-
adenylylsulfate pH 8.0, 30°C, wild-type enzyme Penicillium chrysogenum
2.7.7.4 0.0005
-
adenylylsulfate pH 8.0, 30°C, truncated mutant enzyme del396-573 Penicillium chrysogenum
2.7.7.4 0.0092
-
diphosphate pH 8.0, 30°C, wild-type enzyme Penicillium chrysogenum
2.7.7.4 0.025
-
diphosphate pH 8.0, 30°C, truncated mutant enzyme del396-573 Penicillium chrysogenum
2.7.7.4 0.027
-
ATP pH 8.0, 30°C, molybdolysis, truncated mutant enzyme del396-573 Penicillium chrysogenum
2.7.7.4 0.027
-
ATP pH 8.0, 30°C, molybdolysis, wild-type enzyme Penicillium chrysogenum
2.7.7.4 0.076
-
MoO42- pH 8.0, 30°C, wild-type enzyme Penicillium chrysogenum
2.7.7.4 0.21
-
ATP pH 8.0, 30°C, synthesis of adenylylsulfate, wild-type enzyme Penicillium chrysogenum
2.7.7.4 0.29
-
sulfate pH 8.0, 30°C, wild-type enzyme Penicillium chrysogenum
2.7.7.4 0.53
-
MoO42- pH 8.0, 30°C, truncated mutant enzyme del396-573 Penicillium chrysogenum
2.7.7.4 2.6
-
ATP pH 8.0, 30°C, synthesis of adenylylsulfate, truncated mutant enzyme del396-573 Penicillium chrysogenum
2.7.7.4 3.6
-
sulfate pH 8.0, 30°C, truncated mutant enzyme del396-573 Penicillium chrysogenum

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.4 Penicillium chrysogenum Q12650
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.4 ATP + MoO42-
-
Penicillium chrysogenum AMP + adenylylmolybdate
-
r
2.7.7.4 ATP + sulfate
-
Penicillium chrysogenum diphosphate + adenylylsulfate
-
?

Subunits

EC Number Subunits Comment Organism
2.7.7.4 hexamer wild-type enzyme Penicillium chrysogenum
2.7.7.4 monomer 1 * 46000,truncated mutant enzyme del396-573, SDS-PAGE Penicillium chrysogenum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.4 45
-
t1/2 for mutant enzyme del396-573: 1.5 min Penicillium chrysogenum
2.7.7.4 50
-
t1/2 for mutant enzyme del396-573: 0.3 min, wild-type enzyme is stable for more than 2 h Penicillium chrysogenum
2.7.7.4 62
-
t1/2 for wild-type enzyme: 1.5 min Penicillium chrysogenum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.4 1.8
-
ATP pH 8.0, 30°C, synthesis of adenylylsulfate, truncated mutant enzyme del396-573 Penicillium chrysogenum
2.7.7.4 1.8
-
sulfate pH 8.0, 30°C, truncated mutant enzyme del396-573 Penicillium chrysogenum
2.7.7.4 10.8
-
ATP pH 8.0, 30°C, synthesis of adenylylsulfate, wild-type enzyme Penicillium chrysogenum
2.7.7.4 10.8
-
sulfate pH 8.0, 30°C, wild-type enzyme Penicillium chrysogenum
2.7.7.4 13.7
-
ATP pH 8.0, 30°C, molybdolysis, truncated mutant enzyme del396-573 Penicillium chrysogenum
2.7.7.4 13.7
-
MoO42- pH 8.0, 30°C,truncated mutant enzyme del396-573 Penicillium chrysogenum
2.7.7.4 24.4
-
ATP pH 8.0, 30°C, molybdolysis, wild-type enzyme Penicillium chrysogenum
2.7.7.4 24.4
-
MoO42- pH 8.0, 30°C, wild-type enzyme Penicillium chrysogenum
2.7.7.4 46.6
-
adenylylsulfate pH 8.0, 30°C, truncated mutant enzyme del396-573 Penicillium chrysogenum
2.7.7.4 46.6
-
adenylylsulfate pH 8.0, 30°C, wild-type enzyme Penicillium chrysogenum
2.7.7.4 73.3
-
diphosphate pH 8.0, 30°C, truncated mutant enzyme del396-573 Penicillium chrysogenum
2.7.7.4 73.3
-
diphosphate pH 8.0, 30°C, wild-type enzyme Penicillium chrysogenum