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Literature summary extracted from

  • Jez, J.M.; Cahoon, R.E.
    Kinetic mechanism of glutathione synthetase from Arabidopsis thaliana (2004), J. Biol. Chem., 279, 42726-42731.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.3 overexpression in an Escherichia coli expression system Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.3 ADP product inhibition Arabidopsis thaliana
6.3.2.3 glutathione product inhibition Arabidopsis thaliana
6.3.2.3 phosphate product inhibition Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.3 0.039
-
 gamma-Glu-L-Cys 25°C, pH 7.5 Arabidopsis thaliana
6.3.2.3 0.057
-
 ATP 25°C, pH 7.5 Arabidopsis thaliana
6.3.2.3 1.51
-
 Gly 25°C, pH 7.5 Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.3 Arabidopsis thaliana P46416
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.3 recombinant enzyme Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.3 ATP + gamma-Glu-L-Cys + Gly random ter-reactant mechanism, ADP is the last product released Arabidopsis thaliana ADP + phosphate + glutathione
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.2.3 12.1
-
 ATP 25°C, pH 7.5 Arabidopsis thaliana
6.3.2.3 12.2
-
 gamma-Glu-L-Cys 25°C, pH 7.5 Arabidopsis thaliana
6.3.2.3 12.6
-
 Gly 25°C, pH 7.5 Arabidopsis thaliana

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.2.3 0.77
-
 ADP 25°C, pH 7.5, with glycine as varied substrate Arabidopsis thaliana
6.3.2.3 1.89
-
 ADP 25°C, pH 7.5, with ATP as varied substrate Arabidopsis thaliana
6.3.2.3 2.67
-
 ADP 25°C, pH 7.5, with gamma-Glu-L-Cys as varied substrate Arabidopsis thaliana
6.3.2.3 16.6
-
 glutathione 25°C, pH 7.5, with gamma-Glu-L-Cys as varied substrate Arabidopsis thaliana
6.3.2.3 22.7
-
 glutathione 25°C, pH 7.5, with glycine as varied substrate Arabidopsis thaliana
6.3.2.3 25.1
-
 phosphate 25°C, pH 7.5, with ATP as varied substrate Arabidopsis thaliana
6.3.2.3 35.4
-
 glutathione 25°C, pH 7.5, with ATP as varied substrate Arabidopsis thaliana
6.3.2.3 47.3
-
 phosphate 25°C, pH 7.5, with glycine as varied substrate Arabidopsis thaliana
6.3.2.3 69.1
-
 phosphate 25°C, pH 7.5, with gamma-Glu-L-Cys as varied substrate Arabidopsis thaliana