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Literature summary extracted from

  • MacPherson, M.R.; Lohmann, S.M.; Davies, S.A.
    Analysis of Drosophila cGMP-dependent protein kinases and assessment of their in vivo roles by targeted expression in a renal transporting epithelium (2004), J. Biol. Chem., 279, 40026-40034.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.12 cGMP stimulates DG2P1 and DG2P2, and DG1, the latter maximally at 20 mM Drosophila melanogaster

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.12 in vitro transcription of DG2P1 and DG2P2, and DG1 by RT-PCR of cDNAs from tubule cells, targeted expression of DG2P1 and DG2P2, and DG1 in malpigian tubule using the UAS/GAL4 system, differential localization of all 3 enzyme forms in transgenic flies Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.12 additional information construction of transgenic flies by targeted expression of DG2P1 and DG2P2, and DG1 in malpigian tubule using the UAS/GAL4 system, differential localization of all 3 enzyme forms in transgenic flies, phenotypes, overview Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.12 Rp-8-pCPT-cGMP i.e. 8-(4-chlorophenylthio)guanosine-3',5'-cyclic monophosphorothionate Rp isomer, inhibits DG2P1 and DG2P2 at 0.01 and 0.1 mM, respectively Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.12 cytosol DG1 Drosophila melanogaster 5829
-
2.7.11.12 plasma membrane DG2P1 and DG2P2 Drosophila melanogaster 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.12 Mg2+ required by DG2P1 and DG2P2, and DG1 Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.12 65000
-
 x * 65000, recombinant DG1, SDS-PAGE, x * 84000, recombinant DG2P1 and DG2P2, SDS-PAGE Drosophila melanogaster
2.7.11.12 84000
-
 x * 65000, recombinant DG1, SDS-PAGE, x * 84000, recombinant DG2P1 and DG2P2, SDS-PAGE Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.12 additional information Drosophila melanogaster DG2P1 and DG2P2 are involved in behaviour and epithelial transport ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.12 Drosophila melanogaster
-
 gene dg2 or for encoding two enzyme forms DG2P1 and DG2P2, and gene dg1 encoding enzyme form DG1
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.12 phosphoprotein autophosphorylation by DG1 Drosophila melanogaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.12 epithelium
-
 Drosophila melanogaster
-
2.7.11.12 head of the fly Drosophila melanogaster
-
2.7.11.12 malpighian tubule
-
 Drosophila melanogaster
-
2.7.11.12 SCHNEIDER-2 cell
-
 Drosophila melanogaster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.12 ATP + histone DG1 Drosophila melanogaster ADP + phosphorylated histone
-
 ?
2.7.11.12 ATP + RKRSRAE cGK-specific peptide substrate Drosophila melanogaster ADP + RKRS(P)RAE
-
 ?
2.7.11.12 additional information DG2P1 and DG2P2 are involved in behaviour and epithelial transport Drosophila melanogaster ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.12 ? x * 65000, recombinant DG1, SDS-PAGE, x * 84000, recombinant DG2P1 and DG2P2, SDS-PAGE Drosophila melanogaster

Synonyms

EC Number Synonyms Comment Organism
2.7.11.12 CGK
-
 Drosophila melanogaster
2.7.11.12 DG2P1
-
 Drosophila melanogaster
2.7.11.12 DG2P2
-
 Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.12 7.5
-
 assay at Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.12 ATP
-
 Drosophila melanogaster