BRENDA - Enzyme Database

Cofactor-independent phosphoglycerate mutase has an essential role in Caenorhabditis elegans and is conserved in parasitic nematodes

Zhang, Y.; Foster, J.M.; Kumar, S.; Fougere, M.; Carlow, C.K.; J. Biol. Chem. 279, 37185-37190 (2004)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.4.2.12
expression in Escherichia coli with His6-tag
Brugia malayi
5.4.2.12
expression in Escherichia coli with His6-tag
Caenorhabditis elegans
Engineering
EC Number
Amino acid exchange
Commentary
Organism
5.4.2.12
additional information
reduction of enzyme activity by RNA interference leads to embroynic lethality, larval lethylity, and abnormal body morphology
Caenorhabditis elegans
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.2.12
additional information
not inhibitory: vanadate
Brugia malayi
5.4.2.12
additional information
not inhibitory: vanadate
Caenorhabditis elegans
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.2.12
60000
-
x * 60000, SDS-PAGE
Brugia malayi
5.4.2.12
60000
-
x * 60000, SDS-PAGE
Caenorhabditis elegans
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.2.12
Brugia malayi
Q4VWF8
-
-
5.4.2.12
Caenorhabditis elegans
G5EFZ1
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
5.4.2.12
40
-
pH 7.0, 30░C, formation of 2-phospho-D-glycerate
Caenorhabditis elegans
5.4.2.12
72
-
pH 7.0, 30░C, formation of 3-phospho-D-glycerate
Caenorhabditis elegans
5.4.2.12
93
-
pH 7.0, 30░C, formation of 2-phospho-D-glycerate
Brugia malayi
5.4.2.12
256
-
pH 7.0, 30░C, formation of 3-phospho-D-glycerate
Brugia malayi
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.12
2-phospho-D-glycerate
-
662259
Brugia malayi
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
2-phospho-D-glycerate
-
662259
Caenorhabditis elegans
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
662259
Brugia malayi
2-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
662259
Caenorhabditis elegans
2-phospho-D-glycerate
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
5.4.2.12
?
x * 60000, SDS-PAGE
Brugia malayi
5.4.2.12
?
x * 60000, SDS-PAGE
Caenorhabditis elegans
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.12
additional information
2,3-bisphosphoglycerate-independent phosphoglycerate mutase
Brugia malayi
5.4.2.12
additional information
2,3-bisphosphoglycerate independent phosphoglycerate mutase
Caenorhabditis elegans
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.12
expression in Escherichia coli with His6-tag
Brugia malayi
5.4.2.12
expression in Escherichia coli with His6-tag
Caenorhabditis elegans
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.12
additional information
2,3-bisphosphoglycerate-independent phosphoglycerate mutase
Brugia malayi
5.4.2.12
additional information
2,3-bisphosphoglycerate independent phosphoglycerate mutase
Caenorhabditis elegans
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
5.4.2.12
additional information
reduction of enzyme activity by RNA interference leads to embroynic lethality, larval lethylity, and abnormal body morphology
Caenorhabditis elegans
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.2.12
additional information
not inhibitory: vanadate
Brugia malayi
5.4.2.12
additional information
not inhibitory: vanadate
Caenorhabditis elegans
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.2.12
60000
-
x * 60000, SDS-PAGE
Brugia malayi
5.4.2.12
60000
-
x * 60000, SDS-PAGE
Caenorhabditis elegans
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
5.4.2.12
40
-
pH 7.0, 30░C, formation of 2-phospho-D-glycerate
Caenorhabditis elegans
5.4.2.12
72
-
pH 7.0, 30░C, formation of 3-phospho-D-glycerate
Caenorhabditis elegans
5.4.2.12
93
-
pH 7.0, 30░C, formation of 2-phospho-D-glycerate
Brugia malayi
5.4.2.12
256
-
pH 7.0, 30░C, formation of 3-phospho-D-glycerate
Brugia malayi
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.12
2-phospho-D-glycerate
-
662259
Brugia malayi
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
2-phospho-D-glycerate
-
662259
Caenorhabditis elegans
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
662259
Brugia malayi
2-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
662259
Caenorhabditis elegans
2-phospho-D-glycerate
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.4.2.12
?
x * 60000, SDS-PAGE
Brugia malayi
5.4.2.12
?
x * 60000, SDS-PAGE
Caenorhabditis elegans