Literature summary extracted from
Hao, G.; Xie, L.; Gross, S.S.
Argininosuccinate synthetase is reversibly inactivated by S-nitrosylation in vitro and in vivo (2004), J. Biol. Chem., 279, 36192-36200.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
6.3.4.5 |
expressed as a C-terminal fusion protein with maltose-binding protein in Escherichia coli |
Homo sapiens |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
6.3.4.5 |
NO |
mediates reversible S-nitrosylation and inactivation of argininosuccinate synthetase in vitro and in lipopolysaccharide-treated cells and mice. S-nitrosylation of Cys132 is noth necessary and sufficient for the inhibition of argininosuccinate synthase by NO donors |
Homo sapiens |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
6.3.4.5 |
ATP + L-citrulline + L-Asp |
Homo sapiens |
the substrate for NO synthesis, L-arginine can be regenerated from the L-citrulline the coproduct of nitric-oxide synthase. This requires the sequential action of two enzymes, argininosuccinate synthetase and argininosuccinate lyase |
AMP + diphosphate + L-argininosuccinate |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
6.3.4.5 |
Homo sapiens |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
6.3.4.5 |
recombinant |
Homo sapiens |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
6.3.4.5 |
ATP + L-citrulline + L-Asp |
the substrate for NO synthesis, L-arginine can be regenerated from the L-citrulline the coproduct of nitric-oxide synthase. This requires the sequential action of two enzymes, argininosuccinate synthetase and argininosuccinate lyase |
Homo sapiens |
AMP + diphosphate + L-argininosuccinate |
- |
? |
|