EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.82 | expression of the enzyme fused to the lacZ reporter gene in several yeast strains and in Escherichia coli strain DH5alpha, expression analysis in presence of inositol and choline in correlation to wild-type and mutated promotor | Saccharomyces cerevisiae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.82 | Inositol | decreases gene EKI1 expression, involving the transcription factors Ino2p, Ino4p, and Opi1p, the UASINO element of the EKI promotor is required for the repressive regulation, the inhibitory effect on the enzyme is correlated with a decrease of ethanolamine incorporation into CDP-ethanolamine pathway intermediates and into phosphatidylethanolamine and phosphatidylcholine | Saccharomyces cerevisiae | |
2.7.1.82 | additional information | enzyme expression is not affected by addition of choline | Saccharomyces cerevisiae |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.82 | Mg2+ | - |
Saccharomyces cerevisiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.82 | ATP + ethanolamine | Saccharomyces cerevisiae | the enzyme and the phospholipid phosphatidylethanolamine biosynthesis are regulated by inositol, Kennedy pathway overview | ADP + O-phosphoethanolamine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.82 | Saccharomyces cerevisiae | - |
gene EKI1 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.82 | ATP + ethanolamine | - |
Saccharomyces cerevisiae | ADP + O-phosphoethanolamine | - |
? | |
2.7.1.82 | ATP + ethanolamine | the enzyme and the phospholipid phosphatidylethanolamine biosynthesis are regulated by inositol, Kennedy pathway overview | Saccharomyces cerevisiae | ADP + O-phosphoethanolamine | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.82 | 30 | - |
assay at | Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.82 | 8.5 | - |
assay at | Saccharomyces cerevisiae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.82 | ATP | - |
Saccharomyces cerevisiae |