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Literature summary extracted from

  • Kersting, M.C.; Choi, H.S.; Carman, G.M.
    Regulation of the yeast EKI1-encoded ethanolamine kinase by inositol and choline (2004), J. Biol. Chem., 279, 35353-35359.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.82 expression of the enzyme fused to the lacZ reporter gene in several yeast strains and in Escherichia coli strain DH5alpha, expression analysis in presence of inositol and choline in correlation to wild-type and mutated promotor Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.82 Inositol decreases gene EKI1 expression, involving the transcription factors Ino2p, Ino4p, and Opi1p, the UASINO element of the EKI promotor is required for the repressive regulation, the inhibitory effect on the enzyme is correlated with a decrease of ethanolamine incorporation into CDP-ethanolamine pathway intermediates and into phosphatidylethanolamine and phosphatidylcholine Saccharomyces cerevisiae
2.7.1.82 additional information enzyme expression is not affected by addition of choline Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.82 Mg2+
-
Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.82 ATP + ethanolamine Saccharomyces cerevisiae the enzyme and the phospholipid phosphatidylethanolamine biosynthesis are regulated by inositol, Kennedy pathway overview ADP + O-phosphoethanolamine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.82 Saccharomyces cerevisiae
-
gene EKI1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.82 ATP + ethanolamine
-
Saccharomyces cerevisiae ADP + O-phosphoethanolamine
-
?
2.7.1.82 ATP + ethanolamine the enzyme and the phospholipid phosphatidylethanolamine biosynthesis are regulated by inositol, Kennedy pathway overview Saccharomyces cerevisiae ADP + O-phosphoethanolamine
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.82 30
-
assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.82 8.5
-
assay at Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.82 ATP
-
Saccharomyces cerevisiae