Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Xu, M.G.; Zhao, M.W.; Wang, E.D.
    Leucyl-tRNA synthetase from the hyperthermophilic bacterium Aquifex aeolicus recognizes minihelices (2004), J. Biol. Chem., 279, 32151-32158.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.4 0.00061
-
 tRNALeu 37°C, pH 7.8 Aquifex aeolicus
6.1.1.4 0.0012
-
 tRNALeuA35G 37°C, pH 7.8 Aquifex aeolicus
6.1.1.4 0.011
-
 tRNALeuA73G 37°C, pH 7.8 Aquifex aeolicus

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.4 Aquifex aeolicus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu
-
 Aquifex aeolicus AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
6.1.1.4 ATP + L-leucine + tRNALeuA35G
-
 Aquifex aeolicus AMP + diphosphate + L-leucyl-tRNALeuA35G
-
 ?
6.1.1.4 ATP + L-leucine + tRNALeuA73G
-
 Aquifex aeolicus AMP + diphosphate + L-leucyl-tRNALeuA73G
-
 ?
6.1.1.4 additional information aminoacylation of minihelices is strongly dependent on the presence of the A73 identity nucleotide and greatly stimulated by destabilization of the first base pair. Addition of RNA helices that mimic the anticodon domain stimulates minihelixLeu charging by alphabeta-LeuRS indicating possible domain-domain communication. MinihelixLeu cannot be misaminoacylated, perhaps because of the tRNA-independent pretransfer editing activity of alphabeta-LeuRS Aquifex aeolicus ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.4 0.11
-
 tRNALeuA73G 37°C, pH 7.8 Aquifex aeolicus
6.1.1.4 0.31
-
 tRNALeuA35G 37°C, pH 7.8 Aquifex aeolicus
6.1.1.4 0.5
-
 tRNALeu 37°C, pH 7.8 Aquifex aeolicus