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Literature summary extracted from

  • Nandakumar, J.; Ho, C.K.; Lima, C.D.; Shuman, S.
    RNA substrate specificity and structure-guided mutational analysis of bacteriophage T4 RNA ligase 2 (2004), J. Biol. Chem., 279, 31337-31347.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.5.1.3 D135A about 5% of the ligation activity of wild-type enzyme, mutant enzyme displays 4% of the adenylyltransferase activity observed with wild-type enzyme Tequatrovirus T4
6.5.1.3 D292A no ligation activity Tequatrovirus T4
6.5.1.3 D292E about 10% of the ligation activity of wild-type enzyme Tequatrovirus T4
6.5.1.3 D308A about 70% of the ligation activity of wild-type enzyme Tequatrovirus T4
6.5.1.3 E139A about as active as wild-type enzyme in RNA ligation, mutant displays near wild-type adenylyltransferase activity Tequatrovirus T4
6.5.1.3 E295A about as active as wild-type enzyme in RNA ligation Tequatrovirus T4
6.5.1.3 E296A about 10% of the ligation activity of wild-type enzyme Tequatrovirus T4
6.5.1.3 E299A about 80% of the ligation activity of wild-type enzyme Tequatrovirus T4
6.5.1.3 E29A about 90% of the RNA ligation activity of wild-type enzyme, mutant displays near wild-type adenylyltransferase activity Tequatrovirus T4
6.5.1.3 E300A about 50% of the ligation activity of wild-type enzyme Tequatrovirus T4
6.5.1.3 E63A about 80% of the RNA ligation activity of wild-type enzyme, mutant displays near wild-type adenylyltransferase activity Tequatrovirus T4
6.5.1.3 K107A about as active as wild-type enzyme in RNA ligation, mutant displays near wild-type adenylyltransferase activity Tequatrovirus T4
6.5.1.3 K273A about as active as wild-type enzyme in RNA ligation Tequatrovirus T4
6.5.1.3 K314A about as active as wild-type enzyme in RNA ligation Tequatrovirus T4
6.5.1.3 K315A about 90% of the ligation activity of wild-type enzyme Tequatrovirus T4
6.5.1.3 K319A about as active as wild-type enzyme in RNA ligation Tequatrovirus T4
6.5.1.3 K54A about 15% of the RNA ligation activity of wild-type enzyme, mutant displays near wild-type adenylyltransferase activity Tequatrovirus T4
6.5.1.3 N309A about 70% of the ligation activity of wild-type enzyme Tequatrovirus T4
6.5.1.3 Q106A about 20% of the RNA ligation activity of wild-type enzyme, mutant displays near wild-type adenylyltransferase activity Tequatrovirus T4
6.5.1.3 R155A about 35% of the ligation activity of wild-type enzyme, mutant enzyme displays 16% of the adenylyltransferase activity observed with wild-type enzyme Tequatrovirus T4
6.5.1.3 R221A about 60% of the ligation activity of wild-type enzyme, mutant displays near wild-type adenylyltransferase activity Tequatrovirus T4
6.5.1.3 R266A no ligation activity Tequatrovirus T4
6.5.1.3 R266K about 50% of the ligation activity of wild-type enzyme Tequatrovirus T4
6.5.1.3 R266Q no ligation activity Tequatrovirus T4
6.5.1.3 R33A no ligation activity, mutant enzyme displays 3% of the adenylyltransferase activity observed with wild-type enzyme Tequatrovirus T4
6.5.1.3 S170A about 5% of the ligation activity of wild-type enzyme, mutant enzyme displays 8% of the adenylyltransferase activity observed with wild-type enzyme Tequatrovirus T4
6.5.1.3 S272A about 50% of the ligation activity of wild-type enzyme Tequatrovirus T4
6.5.1.3 S292N about 25% of the ligation activity of wild-type enzyme Tequatrovirus T4
6.5.1.3 W329A about as active as wild-type enzyme in RNA ligation Tequatrovirus T4
6.5.1.3 Y136A about 95% of the ligation activity of wild-type enzyme, mutant displays near wild-type adenylyltransferase activity Tequatrovirus T4
6.5.1.3 Y5A about 10% of the RNA ligation activity of wild-type enzyme, mutant enzyme displays 39% of the adenylyltransferase activity observed with wild-type enzyme Tequatrovirus T4

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.3 Mg2+ Mg2+ or Mn2+ required Tequatrovirus T4
6.5.1.3 Mn2+ Mg2+ or Mn2+ required Tequatrovirus T4

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.3 Tequatrovirus T4
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.3 ATP + (ribonucleotide)n + (ribonucleotide)m neither dATP, GTP, CTP, nor UTP can substitute for ATP. RNA ligation at a 3'-OH/5'-PO4 nick in a double-stranded RNA or an RNA-DNA hybrid Tequatrovirus T4 AMP + diphosphate + (ribonucleotide)n+m
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.3 RNA ligase 2
-
 Tequatrovirus T4
6.5.1.3 Rnl2
-
 Tequatrovirus T4