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Literature summary extracted from

  • Xie, M.; Smith, J.L.; Ding, Z.; Zhang, D.; Cornell, R.B.
    Membrane binding modulates the quaternary structure of CTP:phosphocholine cytidylyltransferase (2004), J. Biol. Chem., 279, 28817-28825.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.15 Lipids regulation of activity by reversible association with membranes containing anionic lipids Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.15 native and mutant enzyme expressed as His-tag fusion proteins in COS-1 cells Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.15 C113S no significant effect of activity Rattus norvegicus
2.7.7.15 C139S no significant effect of activity Rattus norvegicus
2.7.7.15 C354S no significant effect of activity Rattus norvegicus
2.7.7.15 C359S no significant effect of activity Rattus norvegicus
2.7.7.15 C37S unable to form dimers Rattus norvegicus
2.7.7.15 C68S no significant effect of activity Rattus norvegicus
2.7.7.15 C73S no significant effect of activity Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.15 membrane enzyme is activated when bound to vesicles Rattus norvegicus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.15 42000
-
 2 * 42000, SDS-PAGE Rattus norvegicus
2.7.7.15 80000 110000 diffuse band in SDS-PAGE using chemically cross-linked protein Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.15 CTP + choline phosphate Rattus norvegicus rate limiting enzyme for the synthesis of phosphatidylcholine diphosphate + CDP-choline
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.15 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.15 recombinant enzymes using His-tag Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.15 CTP + choline phosphate
-
 Rattus norvegicus diphosphate + CDP-choline
-
 ?
2.7.7.15 CTP + choline phosphate rate limiting enzyme for the synthesis of phosphatidylcholine Rattus norvegicus diphosphate + CDP-choline
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.15 dimer 2 * 42000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.7.7.15 CCTalpha
-
 Rattus norvegicus