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Literature summary extracted from

  • Smith, S.A.; Tabita, F.R.
    Glycine 176 affects catalytic properties and stability of the Synechococcus sp. strain PCC6301 ribulose-1,5-bisphosphate carboxylase/oxygenase (2004), J. Biol. Chem., 279, 25632-25637.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.39 G176A 2.7fold decrease in turnover number for D-ribulose 1,5-bisphosphate, KM-value for D-ribulose 1,5-bisphosphate is nearly identical to wild-type value Synechococcus sp.
4.1.1.39 G176D 11.7fold decrease in turnover number for D-ribulose 1,5-bisphosphate, 2.2fold increase in KM-value for D-ribulose 1,5-bisphosphate Synechococcus sp.
4.1.1.39 G176N 8.75fold decrease in turnover number for D-ribulose 1,5-bisphosphate, 2fold increase in KM-value for D-ribulose 1,5-bisphosphate Synechococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.39 0.04
-
 D-ribulose 1,5-bisphosphate mutant enzyme G176A Synechococcus sp.
4.1.1.39 0.0447
-
 D-ribulose 1,5-bisphosphate wild-type enzyme Synechococcus sp.
4.1.1.39 0.0916
-
 D-ribulose 1,5-bisphosphate mutant enzyme G176N Synechococcus sp.
4.1.1.39 0.1
-
 D-ribulose 1,5-bisphosphate mutant enzyme G176D Synechococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.39 Synechococcus sp.
-
-
-
4.1.1.39 Synechococcus sp. PCC6301
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.39 D-ribulose 1,5-bisphosphate + CO2 + H2O
-
 Synechococcus sp. 3-phospho-D-glycerate
-
 ?
4.1.1.39 D-ribulose 1,5-bisphosphate + CO2 + H2O
-
 Synechococcus sp. PCC6301 3-phospho-D-glycerate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.39 ribulose-1,5-bisphosphate carboxylase/oxygenase
-
 Synechococcus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.39 0.3
-
 D-ribulose 1,5-bisphosphate mutant enzyme G176D Synechococcus sp.
4.1.1.39 0.4
-
 D-ribulose 1,5-bisphosphate mutant enzyme G176N Synechococcus sp.
4.1.1.39 1.3
-
 D-ribulose 1,5-bisphosphate mutant enzyme G176A Synechococcus sp.
4.1.1.39 3.5
-
 D-ribulose 1,5-bisphosphate wild-type enzyme Synechococcus sp.