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Literature summary extracted from

  • Ahn, Y.O.; Mizutani, M.; Saino, H.; Sakata, K.
    Furcatin hydrolase from Viburnum furcatum Blume is a novel disaccharide-specific acuminosidase in glycosyl hydrolase family 1 (2004), J. Biol. Chem., 279, 23405-23414.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.161 expression in Escherichia coli Viburnum furcatum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.161 2.2
-
 furcatin pH 6.0, 37°C Viburnum furcatum
3.2.1.161 5.1
-
 p-nitrophenyl beta-primeveroside pH 6.0, 37°C Viburnum furcatum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.161 chloroplast
-
 Viburnum furcatum 9507
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.161 Viburnum furcatum Q75W17 Blume
-
3.2.1.161 Viburnum furcatum Blume Q75W17 Blume
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.161
-
 Viburnum furcatum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.161 leaf
-
 Viburnum furcatum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.161 42.2
-
-
 Viburnum furcatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.161 2-phenylethyl beta-primeveroside + H2O 14.7% of the activity with furcatin Viburnum furcatum 2-phenylethanol + 6-O-beta-D-xylopyranosyl-beta-D-glucopyranose
-
 ?
3.2.1.161 2-phenylethyl beta-primeveroside + H2O 14.7% of the activity with furcatin Viburnum furcatum Blume 2-phenylethanol + 6-O-beta-D-xylopyranosyl-beta-D-glucopyranose
-
 ?
3.2.1.161 4-nitrophenyl beta-D-glucopyranoside + H2O 3.33% of the activity with furcatin Viburnum furcatum 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.161 4-nitrophenyl beta-D-glucopyranoside + H2O 3.33% of the activity with furcatin Viburnum furcatum Blume 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.161 beta-allylphenyl-beta-D-glucopyranoside + H2O 43.3% of the activity with furcatin Viburnum furcatum 4-allylphenol + beta-D-glucopyranose
-
 ?
3.2.1.161 beta-allylphenyl-beta-D-glucopyranoside + H2O 43.3% of the activity with furcatin Viburnum furcatum Blume 4-allylphenol + beta-D-glucopyranose
-
 ?
3.2.1.161 furcatin + H2O
-
 Viburnum furcatum 4-allylphenol + beta-D-apiofuranosyl-(1-6)-beta-D-glucopyranose
-
 ?
3.2.1.161 furcatin + H2O
-
 Viburnum furcatum Blume 4-allylphenol + beta-D-apiofuranosyl-(1-6)-beta-D-glucopyranose
-
 ?
3.2.1.161 p-nitrophenyl beta-gentiobioside + H2O 4.11% of the activity with furcatin Viburnum furcatum 4-nitrophenol + beta-gentiobiose
-
 ?
3.2.1.161 p-nitrophenyl beta-gentiobioside + H2O 4.11% of the activity with furcatin Viburnum furcatum Blume 4-nitrophenol + beta-gentiobiose
-
 ?
3.2.1.161 p-nitrophenyl beta-primeveroside + H2O 87.3% of the activity with furcatin Viburnum furcatum 4-nitrophenol + 6-O-beta-D-xylopyranosyl-beta-D-glucopyranose
-
 ?
3.2.1.161 vicianin + H2O 4.99% of the activity with furcatin Viburnum furcatum mandelonitrile + 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.161 furcatin hydrolase
-
 Viburnum furcatum
3.2.1.161 isoflavonoid 7-O-beta-apiosyl-glucoside beta-glucosidase
-
 Viburnum furcatum
3.2.1.161 isoflavonoid-7-O-beta[D-apiofuranosyl-(1->6)-beta-D-glucoside] disaccharidase
-
 Viburnum furcatum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.161 40
-
-
 Viburnum furcatum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.161 50
-
 stable below Viburnum furcatum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.161 5
-
-
 Viburnum furcatum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.161 4 10 recombinant enzyme is active over the range Viburnum furcatum