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Literature summary extracted from

  • Yuan, C.; Kent, C.
    Identification of critical residues of ATP kinase A2 from Caenorhabditis elegans (2004), J. Biol. Chem., 279, 17801-17809.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.32 native and mutant enzymes expressed as His-tag fusion protein in Sf9 cells Caenorhabditis elegans

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.32 C252S slightly increased turnover Caenorhabditis elegans
2.7.1.32 D255A no activity detectable Caenorhabditis elegans
2.7.1.32 D255E activity almost completely abolished Caenorhabditis elegans
2.7.1.32 D255N no activity detectable Caenorhabditis elegans
2.7.1.32 D301A no activity detectable Caenorhabditis elegans
2.7.1.32 D301E no activity detectable Caenorhabditis elegans
2.7.1.32 D301N no activity detectable Caenorhabditis elegans
2.7.1.32 D313A low expression level Caenorhabditis elegans
2.7.1.32 E125A reduced activity Caenorhabditis elegans
2.7.1.32 E125D increased turnover Caenorhabditis elegans
2.7.1.32 E125Q reduced activity Caenorhabditis elegans
2.7.1.32 E134A slightly increased turnover Caenorhabditis elegans
2.7.1.32 E151A slightly increased turnover Caenorhabditis elegans
2.7.1.32 E303A strongly reduced activity, increased Km for both substrates, strongly increased Km for Mg2+ Caenorhabditis elegans
2.7.1.32 E303D reduced activity Caenorhabditis elegans
2.7.1.32 E303Q reduced activity Caenorhabditis elegans
2.7.1.32 E320A slightly increased turnover Caenorhabditis elegans
2.7.1.32 H181A low expression level Caenorhabditis elegans
2.7.1.32 H253A reduced activity Caenorhabditis elegans
2.7.1.32 N260A activity almost completely abolished, strongly increased Km for Mg2+ Caenorhabditis elegans
2.7.1.32 N308A reduced activity Caenorhabditis elegans
2.7.1.32 N308D slightly increased turnover Caenorhabditis elegans
2.7.1.32 N308Q reduced activity Caenorhabditis elegans
2.7.1.32 N354A strongly reduced activity, only minor effect on Km for both substrates Caenorhabditis elegans
2.7.1.32 N87A reduced activity Caenorhabditis elegans
2.7.1.32 R111A reduced activity Caenorhabditis elegans
2.7.1.32 R149A reduced activity Caenorhabditis elegans
2.7.1.32 R149K slightly increased turnover Caenorhabditis elegans
2.7.1.32 S85T reduced activity Caenorhabditis elegans
2.7.1.32 S86A strongly reduced activity, increased Km for ATP Caenorhabditis elegans
2.7.1.32 W387A strongly reduced activity, only minor effect on Km for both substrates Caenorhabditis elegans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.32 Ca2+
-
 Caenorhabditis elegans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.32 0.4
-
 choline N260A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 0.7
-
 choline R149A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 0.7
-
 choline S85T mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 0.8
-
 choline E125D mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 0.8
-
 choline N308Q mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 1
-
 ATP C252S mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 1
-
 choline C252S mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 1
-
 choline N308D mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 1
-
 ATP N308Q mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 1
-
 choline N354A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 1
-
 choline wild type enzyme including His-tag, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 1.1
-
 choline E125A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 1.2
-
 ATP N354A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 1.2
-
 choline R149K mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 1.2
-
 choline W387A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 1.3
-
 ATP D255D mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 1.5
-
 choline N308A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 1.5
-
 ATP N308D mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 1.6
-
 choline E125Q mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 1.6
-
 choline H253A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 1.6
-
 choline wild type enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 1.7
-
 ATP E134A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 1.7
-
 ATP N87A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 1.7
-
 choline R111A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 1.7
-
 ATP wild type enzyme including His-tag, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 1.8
-
 choline E134A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 1.8
-
 choline N87A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 1.9
-
 ATP H253A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 1.9
-
 ATP S85T mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 2
-
 ATP N308A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 2.2
-
 ATP E125D mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 2.2
-
 choline E151A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 2.2
-
 ATP R149A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 2.2
-
 ATP W387A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 2.3
-
 ATP R149K mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 2.4
-
 ATP wild type enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 2.5
-
 ATP S86A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 3
-
 choline E320A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 3.1
-
 ATP N260A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 3.2
-
 ATP E125A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 3.4
-
 choline E303Q mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 3.6
-
 choline E303D mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 4.2
-
 ATP E125Q mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 4.5
-
 ATP E151A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 5
-
 choline S86A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 5.4
-
 choline D255D mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 5.4
-
 ATP E303A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 6.9
-
 ATP E303D mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 7
-
 ATP R111A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 8.8
-
 ATP E303Q mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 10
-
 choline E303A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 45
-
 ATP E320A mutant enzyme, pH 10, 37°C Caenorhabditis elegans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.32 Mg2+
-
 Caenorhabditis elegans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.32 51000
-
 2 * 51000, recombinant enzyme including His-tag, SDS-PAGE, native mass by gel filtration Caenorhabditis elegans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.32 ATP + choline Caenorhabditis elegans initial step of the CDP-choline pathway, that yields phosphatidylcholine as final product, involved in mitogenic signaling transduction ADP + O-phosphocholine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.32 Caenorhabditis elegans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.32 recombinant enzymes using His-tag Caenorhabditis elegans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.32 ATP + choline
-
 Caenorhabditis elegans ADP + O-phosphocholine
-
 ?
2.7.1.32 ATP + choline initial step of the CDP-choline pathway, that yields phosphatidylcholine as final product, involved in mitogenic signaling transduction Caenorhabditis elegans ADP + O-phosphocholine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.32 dimer 2 * 51000, recombinant enzyme including His-tag, SDS-PAGE, native mass by gel filtration Caenorhabditis elegans

Synonyms

EC Number Synonyms Comment Organism
2.7.1.32 More at least four genes encoding active choline kinase Caenorhabditis elegans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.32 0.24
-
 choline N260A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 0.25
-
 choline D255D mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 2.4
-
 choline W387A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 2.6
-
 choline N354A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 7
-
 choline E303A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 9.8
-
 choline S86A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 20.8
-
 choline N308A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 21
-
 choline E125A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 27.1
-
 choline R111A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 29.7
-
 choline N87A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 31.4
-
 choline E125Q mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 35.7
-
 choline S85T mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 37.5
-
 choline N308Q mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 38.7
-
 choline E303D mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 38.9
-
 choline R149A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 42.9
-
 choline H253A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 58.7
-
 choline E303Q mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 72.3
-
 choline wild type enzyme including His-tag, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 74
-
 choline wild type enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 79.9
-
 choline E320A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 84.3
-
 choline C252S mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 84.6
-
 choline N308D mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 87.9
-
 choline R149K mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 89.2
-
 choline E151A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 97.3
-
 choline E134A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.7.1.32 130
-
 choline E125D mutant enzyme, pH 10, 37°C Caenorhabditis elegans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.32 10
-
-
 Caenorhabditis elegans