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Literature summary extracted from

  • Nomaguchi, M.; Teramoto, T.; Yu, L.; Markoff, L.; Padmanabhan, R.
    Requirements for West Nile virus (-)- and (+)-strand subgenomic RNA synthesis in vitro by the viral RNA-dependent RNA polymerase expressed in Escherichia coli (2004), J. Biol. Chem., 279, 12141-12151.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.48 additional information the 5'-cap inhibits the (-)-strand RNA synthesis from the 3'-fold-back structure of (+)-strand RNA template without affecting the de novo synthesis of RNA West Nile virus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 West Nile virus
-
 expressed in Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.48
-
 West Nile virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn both 5'- and 3'-terminal regions of the (+)-strand RNA template including the wild type cyclization motifs are important for RNA synthesis. However, the 3'-terminal region of the (-)-strand RNA template alone is sufficient for RNA synthesis. The (+)-strand 5'-cyclization motif is critical for (-)-strand RNA synthesis but neither the (-)-strand 5'- nor 3'-cyclization motif is important for the (+)-strand RNA synthesis. Cyclization of the viral RNA play a role for (-)-strand RNA synthesis but not for (+)-strand RNA synthesis West Nile virus diphosphate + RNAn+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.48 RDRP
-
 West Nile virus
2.7.7.48 RNA-dependent RNA polymerase
-
 West Nile virus
2.7.7.48 WNV NS5
-
 West Nile virus