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Literature summary extracted from

  • Purcarea, C.; Ahuja, A.; Lu, T.; Kovari, L.; Guy, H.I.; Evans, D.R.
    Aquifex aeolicus aspartate transcarbamoylase, an enzyme specialized for the efficient utilization of unstable carbamoyl phosphate at elevated temperature (2003), J. Biol. Chem., 278, 52924-52934.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.3.2 expression in Escherichia coli Aquifex aeolicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.3.2 0.55
-
 Carbamoyl phosphate pH 8.0, 37°C Aquifex aeolicus
2.1.3.2 1
-
 L-aspartate pH 8.0, 37°C Aquifex aeolicus
2.1.3.2 1.12
-
 L-aspartate pH 8.0, 75°C Aquifex aeolicus
2.1.3.2 1.26
-
 Carbamoyl phosphate pH 8.0, 75°C Aquifex aeolicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.2 33557
-
 3 * 37000, SDS-PAGE, recombinant enzyme with His-tag, 3 * 33557, calculated Aquifex aeolicus
2.1.3.2 37000
-
 3 * 37000, SDS-PAGE, recombinant enzyme with His-tag, 3 * 33557, calculated Aquifex aeolicus
2.1.3.2 112800
-
 gel filtration Aquifex aeolicus

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.2 Aquifex aeolicus
-
 expression in Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.2 recombinant enzyme Aquifex aeolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Aquifex aeolicus phosphate + N-carbamoyl-L-aspartate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.2 More channeling and transient complex formation between enzyme and carbamoyl phosphate synthetase Aquifex aeolicus
2.1.3.2 trimer 3 * 37000, SDS-PAGE, recombinant enzyme with His-tag, 3 * 33557, calculated Aquifex aeolicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.3.2 80
-
 stable up to Aquifex aeolicus
2.1.3.2 87
-
 5 min, 50% inactivation Aquifex aeolicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.3.2 5.6
-
 L-aspartate pH 8.0, 37°C Aquifex aeolicus
2.1.3.2 41.7
-
 L-aspartate pH 8.0, 75°C Aquifex aeolicus