Literature summary extracted from

  • Bennett, E.M.; Li, C.; Allan, P.W.; Parker, W.B.; Ealick, S.E.
    Structural basis for substrate specificity of Escherichia coli purine nucleoside phosphorylase (2003), J. Biol. Chem., 278, 47110-47118.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.1 hanging drop vapor diffusion method. Enzyme in complex with nucleoside analogs: 2-fluoroadenosine, 9-beta-D-ribofuranosyl-6-methylthiopurine, 2-fluoro-2'-deoxyadenosine, 9-beta-D-(2-deoxyribofuranosyl)-6-methylthiopurine, adenosine, formycin B, 7-deazaadenosine, 9-beta-D-arabinofuranosyl-adenine, 9-beta-D-xylofuranosyl-adenine or inosine Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.012
-
2-fluoroadenosine pH 7.4, 25°C Escherichia coli
2.4.2.1 0.009
-
9-beta-D-ribofuranosyl-6-methylthiopurine pH 7.4, 25°C Escherichia coli
2.4.2.1 0.023
-
2-fluoro-2'-deoxyadenosine pH 7.4, 25°C Escherichia coli
2.4.2.1 0.096
-
Inosine pH 7.4, 25°C Escherichia coli
2.4.2.1 0.046
-
adenosine pH 7.4, 25°C Escherichia coli
2.4.2.1 0.126
-
9-beta-D-(2-deoxyribofuranosyl)-6-methylthiopurine pH 7.4, 25°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Escherichia coli P0ABP8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 inosine + phosphate
-
Escherichia coli hypoxanthine + alpha-D-ribose 1-phosphate
-
?
2.4.2.1 adenosine + phosphate
-
Escherichia coli adenine + alpha-D-ribose 1-phosphate
-
?
2.4.2.1 2-fluoro-2-deoxyadenosine + phosphate
-
Escherichia coli adenine + 2-fluoro-2-deoxy-alpha-D-ribose 1-phosphate
-
?
2.4.2.1 2-fluoroadenosine + phosphate
-
Escherichia coli 2-fluoroadenine + alpha-D-ribose 1-phosphate
-
?
2.4.2.1 9-beta-D-(2-deoxyribofuranosyl)-6-methylthiopurine + phosphate
-
Escherichia coli 6-methylthiopurine + 2-deoxy-alpha-D-ribose 1-phosphate
-
?
2.4.2.1 9-beta-D-ribofuranosyl-6-methylthiopurine + phosphate
-
Escherichia coli 6-methylthiopurine + alpha-D-ribose 1-phosphate
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.1 3.3
-
adenosine pH 7.4, 25°C Escherichia coli
2.4.2.1 1.1
-
9-beta-D-ribofuranosyl-6-methylthiopurine pH 7.4, 25°C Escherichia coli
2.4.2.1 2.05
-
2-fluoroadenosine pH 7.4, 25°C Escherichia coli
2.4.2.1 9.7
-
9-beta-D-(2-deoxyribofuranosyl)-6-methylthiopurine pH 7.4, 25°C Escherichia coli
2.4.2.1 4
-
Inosine pH 7.4, 25°C Escherichia coli
2.4.2.1 2.77
-
2-fluoro-2'-deoxyadenosine pH 7.4, 25°C Escherichia coli