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Literature summary extracted from

  • Campanini, B.; Raboni, S.; Vaccari, S.; Zhang, L.; Cook, P.F.; Hazlett, T.L.; Mozzarelli, A.; Bettati, S.
    Surface-exposed tryptophan residues are essential for O-acetylserine sulfhydrylase structure, function, and stability (2003), J. Biol. Chem., 278, 37511-37519.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.47 W161Y 2fold increase in Vmax and Km-value of O3-acetyl-L-serine Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.47 W50Y no effect on catalytic rate or affinity of enzyme to first substrate, Km-value for 5-thio-2-nitrobenzoate decrease by 2.7fold Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 0.018
-
 5-thio-2-nitrobenzoate mutant W50Y, pH 7.0, 20°C Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.47 0.049
-
 5-thio-2-nitrobenzoate wild-type, pH 7.0, 20°C Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.47 0.061
-
 5-thio-2-nitrobenzoate mutant W161Y, pH 7.0, 20°C Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.47 0.749
-
 O3-acetyl-L-serine wild-type, pH 7.0, 20°C Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.47 0.774
-
 O3-acetyl-L-serine mutant W50Y, pH 7.0, 20°C Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.47 1.771
-
 O3-acetyl-L-serine mutant W161Y, pH 7.0, 20°C Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Salmonella enterica subsp. enterica serovar Typhimurium
-
 isoform OASS-A
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 O3-acetyl-L-serine + 2-nitro-5-thiobenzoate
-
 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?