Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sakurai, H.; Suzuki, S.; Kawasaki, N.; Nakano, H.; Okazaki, T.; Chino, A.; Doi, T.; Saiki, I.
    Tumor necrosis factor-alpha-induced IKK phosphorylation of NF-kappaB p65 on serine 536 is mediated through the TRAF2, TRAF5, and TAK1 signaling pathway (2003), J. Biol. Chem., 278, 36916-36923.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.10 additional information TNFalpha, TGF-beta-activated kinase 1 TAK1, IKKalpha subunit and IKKbeta subunit induce IKK phosphorylation activity, overview Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.10 expression of mutant IKKbeta and IKKalpha in HeLa cells and in HEK293 cells, expression of His-tagged IKKbeta in Spodoptera frugiperda Sf21 cells via baculovirus infection Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.10 K44M site-directed mutagenesis, inactive IKKalpha mutant Homo sapiens
2.7.11.10 K44M site-directed mutagenesis, inactive IKKbeta mutant Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.10 Bay 11-7082
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.10 cytoplasm
-
 Homo sapiens 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.10 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.10 ATP + [IkappaB protein] Homo sapiens
-
 ADP + [IkappaB phosphoprotein]
-
 ?
2.7.11.10 ATP + [NFkappaB subunit p56] Homo sapiens
-
 ADP + [NFkappaB subunit p56]phosphate
-
 ?
2.7.11.10 additional information Homo sapiens IKK activates and regulates NFkappaB important in intracellular signalling, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.10 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.10 recombinant His-tagged IKKbeta from Sf21 insect cells by nickel affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.10 HEK-293 cell
-
 Homo sapiens
-
2.7.11.10 HeLa cell
-
 Homo sapiens
-
2.7.11.10 JURKAT cell
-
 Homo sapiens
-
2.7.11.10 RAW-264.7 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.10 ATP + [IkappaB protein]
-
 Homo sapiens ADP + [IkappaB phosphoprotein]
-
 ?
2.7.11.10 ATP + [NFkappaB subunit p56]
-
 Homo sapiens ADP + [NFkappaB subunit p56]phosphate
-
 ?
2.7.11.10 ATP + [NFkappaB subunit p56] phosphorylation at serine residues, especially at S536, in the cytoplasm prior to NFkappaB p56 translocation to the nucleus Homo sapiens ADP + [NFkappaB subunit p56]phosphate
-
 ?
2.7.11.10 additional information IKK activates and regulates NFkappaB important in intracellular signalling, overview Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.10 IKKbeta
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.10 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.10 7.6
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.10 ATP
-
 Homo sapiens