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Literature summary extracted from

  • Wada, T.; Kuzuyama, T.; Satoh, S.; Kuramitsu, S.; Yokoyama, S.; Unzai, S.; Tame, J.R.; Park, S.Y.
    Crystal structure of 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, an enzyme in the non-mevalonate pathway of isoprenoid synthesis (2003), J. Biol. Chem., 278, 30022-30027.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.1.148 drug development enzyme is a target for antimicrobial drug development Thermus thermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.148 gene ychB, expression of the selenomethionine-labeled enzyme in Escherichia coli strain B834(DE3) Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.148 purified recombinant selenomethionine-labeled enzyme, hanging drop vapour diffusion method, 20°C, 0.002 ml protein solution containing 2.2 mg/ml protein, 20 ml Tris-HCl, pH 8.0, and 50 mM NaCl, are mixed with 0.001 ml mother liquor containing 33 mM Tris-HCl, pH 8.5, 67 mM sodium acetate, 13% isopropanol, 8% butanol, and 13% PEG 4000, cryoprotection at -173°C in 30% glycerol, X-ray diffraction structure determination and analysis at 1.7 A resolution Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.148 Mg2+ binding site structure, S95, D125, and G94 are important Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.148 ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol Thermus thermophilus the enzyme is essential in the nonmevalonate pathway of isopentenyl diphosphate and dimethylallyl diphosphate biosynthesis, pathway overview ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.1.148 ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol Thermus thermophilus HB8 / ATCC 27634 / DSM 579 the enzyme is essential in the nonmevalonate pathway of isopentenyl diphosphate and dimethylallyl diphosphate biosynthesis, pathway overview ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.148 Thermus thermophilus
-
-
-
2.7.1.148 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.148 recombinant selenomethionine-labeled enzyme from Escherichia coli strain B834(DE3) by ammonium sulfate fractionation, hydrophobic interaction and heparin affinity chromatography, gel filtration, and ultrafiltration Thermus thermophilus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.148 ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol reaction mechanism involves K8 and D125 Thermus thermophilus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.148 ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol the enzyme is essential in the nonmevalonate pathway of isopentenyl diphosphate and dimethylallyl diphosphate biosynthesis, pathway overview Thermus thermophilus ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.1.148 ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol i.e. CDP-ME, the cytosine group plays an essential role in substrate recognition by the enzyme Thermus thermophilus ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.1.148 ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol the enzyme is essential in the nonmevalonate pathway of isopentenyl diphosphate and dimethylallyl diphosphate biosynthesis, pathway overview Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.1.148 ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol i.e. CDP-ME, the cytosine group plays an essential role in substrate recognition by the enzyme Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.1.148 additional information no activity with 4-(adenine 5'-phosphate)-2-C-methyl-D-erythritol and 4-(uridine 5'-phosphate)-2-C-methyl-D-erythritol Thermus thermophilus ?
-
 ?
2.7.1.148 additional information no activity with 4-(adenine 5'-phosphate)-2-C-methyl-D-erythritol and 4-(uridine 5'-phosphate)-2-C-methyl-D-erythritol Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.148 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase
-
 Thermus thermophilus
2.7.1.148 CDP-ME kinase
-
 Thermus thermophilus
2.7.1.148 CMK
-
 Thermus thermophilus
2.7.1.148 More enzyme belongs to the GHMK super family Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.148 50
-
 assay at Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.148 7.5
-
 assay at Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.148 ATP binding site structure, N58 and K83 are important Thermus thermophilus