EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.2.10 | L6A | in contrast to wild-type, quite susceptible to trypsinolysis, 4fold increase in Km-value for reduced H-protein | Escherichia coli |
2.1.2.10 | additional information | N-terminal deletion of 7 amino acids, in contrast to wild-type, quite susceptible to trypsinolysis | Escherichia coli |
2.1.2.10 | T4A | 2fold increase in Km-value for reduced H-protein | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.2.10 | 0.00069 | - |
reduced H-protein | wild-type with His-tag | Escherichia coli | |
2.1.2.10 | 0.0012 | - |
reduced H-protein | mutant T4A, His-tagged | Escherichia coli | |
2.1.2.10 | 0.00237 | - |
reduced H-protein | mutant L6A, His-tagged | Escherichia coli | |
2.1.2.10 | 0.0092 | - |
5,10-methylenetetrahydropteroyltetraglutamate | wild-type with His-tag | Escherichia coli | |
2.1.2.10 | 0.0108 | - |
5,10-methylenetetrahydropteroyltetraglutamate | mutant L6A, His-tagged | Escherichia coli | |
2.1.2.10 | 0.0169 | - |
reduced H-protein | N-terminal deletion mutant, His-tagged | Escherichia coli | |
2.1.2.10 | 0.0174 | - |
5,10-methylenetetrahydropteroyltetraglutamate | mutant T4A, His-tagged | Escherichia coli | |
2.1.2.10 | 0.0382 | - |
5,10-methylenetetrahydropteroyltetraglutamate | N-terminal deletion mutant, His-tagged | Escherichia coli | |
2.1.2.10 | 0.0869 | - |
5,10-methylenetetrahydrofolate | mutant T4A, His-tagged | Escherichia coli | |
2.1.2.10 | 0.0875 | - |
5,10-methylenetetrahydrofolate | mutant L6A, His-tagged | Escherichia coli | |
2.1.2.10 | 0.0881 | - |
5,10-methylenetetrahydrofolate | wild-type with His-tag | Escherichia coli | |
2.1.2.10 | 0.151 | - |
5,10-methylenetetrahydrofolate | N-terminal deletion mutant, His-tagged | Escherichia coli | |
2.1.2.10 | 65.4 | - |
NH4Cl | wild-type with His-tag | Escherichia coli | |
2.1.2.10 | 78.9 | - |
NH4Cl | mutant L6A, His-tagged | Escherichia coli | |
2.1.2.10 | 93.5 | - |
NH4Cl | mutant T4A, His-tagged | Escherichia coli | |
2.1.2.10 | 256 | - |
NH4Cl | N-terminal deletion mutant, His-tagged | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.2.10 | Escherichia coli | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.1.2.10 | [protein]-S8-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3 | formation of a folate-binding cavity via the interaction of enzyme with H-protein | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.10 | reduced H-protein + 5,10-methylene-tetrahydrofolate + NH4Cl | - |
Escherichia coli | ? | - |
? | |
2.1.2.10 | reduced H-protein + 5,10-methylenetetrahydropteroyltetraglutamate + NH4Cl | - |
Escherichia coli | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.2.10 | More | interaction of enzyme with H-protein | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.2.10 | 2.1 | - |
5,10-methylenetetrahydrofolate | N-terminal deletion mutant, His-tagged | Escherichia coli | |
2.1.2.10 | 2.9 | - |
reduced H-protein | N-terminal deletion mutant, His-tagged | Escherichia coli | |
2.1.2.10 | 3.9 | - |
NH4Cl | N-terminal deletion mutant, His-tagged | Escherichia coli | |
2.1.2.10 | 7.8 | - |
5,10-methylenetetrahydrofolate | mutant T4A, His-tagged | Escherichia coli | |
2.1.2.10 | 8.5 | - |
reduced H-protein | mutant T4A, His-tagged | Escherichia coli | |
2.1.2.10 | 9.5 | - |
5,10-methylenetetrahydropteroyltetraglutamate | N-terminal deletion mutant, His-tagged | Escherichia coli | |
2.1.2.10 | 9.6 | - |
NH4Cl | mutant T4A, His-tagged | Escherichia coli | |
2.1.2.10 | 10.3 | - |
5,10-methylenetetrahydrofolate | mutant L6A, His-tagged | Escherichia coli | |
2.1.2.10 | 10.5 | - |
5,10-methylenetetrahydropteroyltetraglutamate | wild-type with His-tag | Escherichia coli | |
2.1.2.10 | 10.6 | - |
5,10-methylenetetrahydropteroyltetraglutamate | mutant L6A, His-tagged | Escherichia coli | |
2.1.2.10 | 11.6 | - |
NH4Cl | mutant L6A, His-tagged | Escherichia coli | |
2.1.2.10 | 11.6 | - |
5,10-methylenetetrahydropteroyltetraglutamate | mutant T4A, His-tagged | Escherichia coli | |
2.1.2.10 | 13.3 | - |
reduced H-protein | mutant L6A, His-tagged | Escherichia coli | |
2.1.2.10 | 18.4 | - |
5,10-methylenetetrahydrofolate | wild-type with His-tag | Escherichia coli | |
2.1.2.10 | 19.4 | - |
reduced H-protein | wild-type with His-tag | Escherichia coli | |
2.1.2.10 | 20.9 | - |
NH4Cl | wild-type with His-tag | Escherichia coli |