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Literature summary extracted from

  • Okamura-Ikeda, K.; Kameoka, N.; Fujiwara, K.; Motokawa, Y.
    Probing the H-protein-induced conformational change and the function of the N-terminal region of Escherichia coli T-protein of the glycine cleavage system by limited proteolysis (2003), J. Biol. Chem., 278, 10067-10072.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.1.2.10 L6A in contrast to wild-type, quite susceptible to trypsinolysis, 4fold increase in Km-value for reduced H-protein Escherichia coli
2.1.2.10 additional information N-terminal deletion of 7 amino acids, in contrast to wild-type, quite susceptible to trypsinolysis Escherichia coli
2.1.2.10 T4A 2fold increase in Km-value for reduced H-protein Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.10 0.00069
-
reduced H-protein wild-type with His-tag Escherichia coli
2.1.2.10 0.0012
-
reduced H-protein mutant T4A, His-tagged Escherichia coli
2.1.2.10 0.00237
-
reduced H-protein mutant L6A, His-tagged Escherichia coli
2.1.2.10 0.0092
-
5,10-methylenetetrahydropteroyltetraglutamate wild-type with His-tag Escherichia coli
2.1.2.10 0.0108
-
5,10-methylenetetrahydropteroyltetraglutamate mutant L6A, His-tagged Escherichia coli
2.1.2.10 0.0169
-
reduced H-protein N-terminal deletion mutant, His-tagged Escherichia coli
2.1.2.10 0.0174
-
5,10-methylenetetrahydropteroyltetraglutamate mutant T4A, His-tagged Escherichia coli
2.1.2.10 0.0382
-
5,10-methylenetetrahydropteroyltetraglutamate N-terminal deletion mutant, His-tagged Escherichia coli
2.1.2.10 0.0869
-
5,10-methylenetetrahydrofolate mutant T4A, His-tagged Escherichia coli
2.1.2.10 0.0875
-
5,10-methylenetetrahydrofolate mutant L6A, His-tagged Escherichia coli
2.1.2.10 0.0881
-
5,10-methylenetetrahydrofolate wild-type with His-tag Escherichia coli
2.1.2.10 0.151
-
5,10-methylenetetrahydrofolate N-terminal deletion mutant, His-tagged Escherichia coli
2.1.2.10 65.4
-
NH4Cl wild-type with His-tag Escherichia coli
2.1.2.10 78.9
-
NH4Cl mutant L6A, His-tagged Escherichia coli
2.1.2.10 93.5
-
NH4Cl mutant T4A, His-tagged Escherichia coli
2.1.2.10 256
-
NH4Cl N-terminal deletion mutant, His-tagged Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.10 Escherichia coli
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.2.10 [protein]-S8-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3 formation of a folate-binding cavity via the interaction of enzyme with H-protein Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.10 reduced H-protein + 5,10-methylene-tetrahydrofolate + NH4Cl
-
Escherichia coli ?
-
?
2.1.2.10 reduced H-protein + 5,10-methylenetetrahydropteroyltetraglutamate + NH4Cl
-
Escherichia coli ?
-
?

Subunits

EC Number Subunits Comment Organism
2.1.2.10 More interaction of enzyme with H-protein Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.10 2.1
-
5,10-methylenetetrahydrofolate N-terminal deletion mutant, His-tagged Escherichia coli
2.1.2.10 2.9
-
reduced H-protein N-terminal deletion mutant, His-tagged Escherichia coli
2.1.2.10 3.9
-
NH4Cl N-terminal deletion mutant, His-tagged Escherichia coli
2.1.2.10 7.8
-
5,10-methylenetetrahydrofolate mutant T4A, His-tagged Escherichia coli
2.1.2.10 8.5
-
reduced H-protein mutant T4A, His-tagged Escherichia coli
2.1.2.10 9.5
-
5,10-methylenetetrahydropteroyltetraglutamate N-terminal deletion mutant, His-tagged Escherichia coli
2.1.2.10 9.6
-
NH4Cl mutant T4A, His-tagged Escherichia coli
2.1.2.10 10.3
-
5,10-methylenetetrahydrofolate mutant L6A, His-tagged Escherichia coli
2.1.2.10 10.5
-
5,10-methylenetetrahydropteroyltetraglutamate wild-type with His-tag Escherichia coli
2.1.2.10 10.6
-
5,10-methylenetetrahydropteroyltetraglutamate mutant L6A, His-tagged Escherichia coli
2.1.2.10 11.6
-
NH4Cl mutant L6A, His-tagged Escherichia coli
2.1.2.10 11.6
-
5,10-methylenetetrahydropteroyltetraglutamate mutant T4A, His-tagged Escherichia coli
2.1.2.10 13.3
-
reduced H-protein mutant L6A, His-tagged Escherichia coli
2.1.2.10 18.4
-
5,10-methylenetetrahydrofolate wild-type with His-tag Escherichia coli
2.1.2.10 19.4
-
reduced H-protein wild-type with His-tag Escherichia coli
2.1.2.10 20.9
-
NH4Cl wild-type with His-tag Escherichia coli