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Literature summary extracted from

  • Finking, R.; Solsbacher, J.; Konz, D.; Schobert, M.; Schäfer, A.; Jahn, D.; Marahiel, M.A.
    Characterization of a new type of phosphopantetheinyl transferase for fatty acid and siderophore synthesis in Pseudomonas aeruginosa (2002), J. Biol. Chem., 277, 50293-50302.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.7 overexpression as a C-terminal His6-tag fusion protein in Escherichia coli Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.7 additional information
-
 additional information
-
 Pseudomonas aeruginosa
2.7.8.7 0.0011
-
 CoA
-
 Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.8.7 27000
-
 C-terminal His6-tag fusion protein, gel filtration Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.7 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.7 recombinant C-terminal His6-tag fusion protein Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.7 CoA + apo-[acyl-carrier protein]
-
 Pseudomonas aeruginosa adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 ?
2.7.8.7 CoA + apo-[peptidyl-carrier protein]
-
 Pseudomonas aeruginosa adenosine 3',5'-bisphosphate + holo-[peptidyl-carrier protein]
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.8.7 monomer
-
 Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
2.7.8.7 PcpS
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.8.7 additional information
-
 additional information
-
 Pseudomonas aeruginosa
2.7.8.7 2.8
-
 CoA
-
 Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.7 6.5
-
 activity with apo-[acyl-carrier protein] Pseudomonas aeruginosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.8.7 5 7 pH 5: less than 27% of maximal activity, pH 7: less than 62% of maximal activity, activity with apo-[acyl-carrier protein] Pseudomonas aeruginosa