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Literature summary extracted from

  • Obst, M.; Oppermann-Sanio, F.B.; Luftmann, H.; Steinbüchel, A.
    Isolation of cyanophycin-degrading bacteria, cloning and characterization of an extracellular cyanophycinase gene (cphE) from Pseudomonas anguilliseptica strain BI (2002), J. Biol. Chem., 277, 25096-25105.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.15.6 expression in Escherichia coli XL1-Blue Pseudomonas anguilliseptica

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.15.6 EDTA 49% inhibition at 60 nM, 46% inhibition at 30 nM Pseudomonas anguilliseptica
3.4.15.6 leupeptin 13% inhibition within the range 0.001 nM to 0.01 nM Pseudomonas anguilliseptica
3.4.15.6 N-bromo-succinimide 100% inhibition at 5 nM, 99% inhibition at 1 nM Pseudomonas anguilliseptica
3.4.15.6 pefabloc i.e. 4-[2-aminoethyl]benzenesulfonyl fluoride, 92% inhibition at 80 nM, 13.6% inhibition at 8 nM Pseudomonas anguilliseptica
3.4.15.6 phenylmethylsulfonyl fluoride 63% inhibition at 10 nM, 28% inhibition at 1 nM Pseudomonas anguilliseptica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.15.6 extracellular
-
 Pseudomonas anguilliseptica
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.15.6 43000
-
 monomer, SDS-PAGE Pseudomonas anguilliseptica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.15.6 [L-Asp(4-Arg)]n + H2O Pseudomonas anguilliseptica
-
 [L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
 ?
3.4.15.6 [L-Asp(4-Arg)]n + H2O Pseudomonas anguilliseptica BI
-
 [L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.15.6 Pseudomonas anguilliseptica Q8KQN8
-
-
3.4.15.6 Pseudomonas anguilliseptica BI Q8KQN8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.15.6 from culture supernatant of Pseudomonas anguilliseptica cells grown in medium containing cyanophycin granules, anion-exchange chromatography and L-arginine-agarose affinity chromatography. In case of heterologous expression in Escherichia coli the enzyme was not secreted and thus, isolation was done from the cell lysate Pseudomonas anguilliseptica

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.4.15.6 in-gel renaturation method Pseudomonas anguilliseptica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.15.6 culture supernatant cells grown in medium containing cyanophycin granules Pseudomonas anguilliseptica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.15.6 [L-Asp(4-Arg)]n + H2O
-
 Pseudomonas anguilliseptica [L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
 ?
3.4.15.6 [L-Asp(4-Arg)]n + H2O
-
 Pseudomonas anguilliseptica BI [L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.15.6 CGPase
-
 Pseudomonas anguilliseptica
3.4.15.6 CphE
-
 Pseudomonas anguilliseptica
3.4.15.6 cyanophycin granule polypeptidase
-
 Pseudomonas anguilliseptica
3.4.15.6 cyanophycinase
-
 Pseudomonas anguilliseptica
3.4.15.6 extracellular CGPase
-
 Pseudomonas anguilliseptica

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.15.6 Pseudomonas anguilliseptica calculated from the sequence
-
 5.92