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Literature summary extracted from

  • Dubois, E.; Scherens, B.; Vierendeels, F.; Ho, M.M.W.; Messenguy, F.; Shears, S.B.
    In Saccharomyces cerevisiae, the inositol polyphosphate kinase activity of Kcs1p is required for resistance to salt stress, cell wall integrity, and vacuolar morphogenesis (2002), J. Biol. Chem., 277, 23755-23763.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.151 gene KCS1, subcloning in Escherichia coli strain XL1-B, overexpression in strain arg82DELTA, expression in strain 12S16cpep4D Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.151 L794A/L801A site-directed mutagenesis, expression in the knockout mutant strain results in activity similar to the wild-type enzyme, phenotype overview Saccharomyces cerevisiae
2.7.1.151 L794A/L801A/L857A/L864A site-directed mutagenesis, expression in the knockout mutant strain results in activity similar to the wild-type enzyme, the mutant strain shows compromised cell wall integrity, phenotype overview Saccharomyces cerevisiae
2.7.1.151 L857A/L864A site-directed mutagenesis, expression in the knockout mutant strain results in activity similar to the wild-type enzyme, phenotype overview Saccharomyces cerevisiae
2.7.1.151 additional information construction of a knockout mutant strain from strain BY4709, the mutant cells contain a fragmented vacuolar compartment, cell growth and cell wall integrity are perturbed, and response to sal stress is altered, a metabolic block in arg82DELTA cells results in the same phenotype, overexpression of the enzyme can partially bypass the metabolic block, overview, expression of full length enzyme and enzyme mutants in the knockout strain, phenotypes overview Saccharomyces cerevisiae
2.7.1.151 S887A/L888A/L889A site-directed mutagenesis, expression in the knockout mutant strain results in activity similar to the wild-type enzyme, the mutant strain shows compromised cell wall integrity, phenotype overview Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.151 Mg2+
-
 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.151 ATP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate Saccharomyces cerevisiae
-
 ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ir
2.7.1.151 ATP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate Saccharomyces cerevisiae
-
 ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ir
2.7.1.151 ATP + 1D-myo-inositol 1,4,5-trisphosphate Saccharomyces cerevisiae
-
 ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ir
2.7.1.151 ATP + 1D-myo-inositol 1,4,5-trisphosphate Saccharomyces cerevisiae
-
 ADP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
-
 ir
2.7.1.151 additional information Saccharomyces cerevisiae the enzyme is required for resistance to salt stress, cell wall integrity, and vascuolar morphogenesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.151 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.151 recombinant enzyme from strain expression in strain 12S16cpep4D by nickel affinity chromatography Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.151 additional information
-
 amount of different inositol phosphates in the knockout mutant strain with or without expression of enzyme and mutants, overview Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.151 ATP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 Saccharomyces cerevisiae ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ir
2.7.1.151 ATP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
-
 Saccharomyces cerevisiae ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ir
2.7.1.151 ATP + 1D-myo-inositol 1,4,5-trisphosphate
-
 Saccharomyces cerevisiae ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ir
2.7.1.151 ATP + 1D-myo-inositol 1,4,5-trisphosphate
-
 Saccharomyces cerevisiae ADP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
-
 ir
2.7.1.151 additional information the enzyme is required for resistance to salt stress, cell wall integrity, and vascuolar morphogenesis Saccharomyces cerevisiae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.151 inositol polyphosphate kinase
-
 Saccharomyces cerevisiae
2.7.1.151 Kcs1p
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.151 37
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.151 7
-
 assay at Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.151 ATP
-
 Saccharomyces cerevisiae