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Literature summary extracted from

  • Grothe, T.; Lenz, R.; Kutchan, T.M.
    Molecular characterization of the salutaridinol 7-O-acetyltransferase involved in morphine biosynthesis in opium poppy Papaver somniferum (2001), J. Biol. Chem., 276, 30717-30723.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.150 synthesis potential application in biotechnological production of morphine alkaloids Papaver somniferum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.150 expressed as His-tag fusion protein in Sf9 insect cells Papaver somniferum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.150 0.009
-
 salutaridinol
-
 Papaver somniferum
2.3.1.150 0.023
-
 nudaurine
-
 Papaver somniferum
2.3.1.150 0.054
-
 acetyl-CoA with salutaridinol as substrate Papaver somniferum
2.3.1.150 0.106
-
 acetyl-CoA with nudaurine as substrate Papaver somniferum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.150 52600
-
 x * 52600, calculated from the deduced amino acid sequence Papaver somniferum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.150 acetyl-CoA + salutaridinol Papaver somniferum involved in morphine biosynthesis CoA + 7-O-acetylsalutaridinol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.150 Papaver somniferum Q94FT4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.150 purification from host organism and recombinant protein using His-tag Papaver somniferum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.150 acetyl-CoA + nudaurine
-
 Papaver somniferum CoA + O-acetylnudaurine
-
 ?
2.3.1.150 acetyl-CoA + salutaridinol
-
 Papaver somniferum CoA + 7-O-acetylsalutaridinol
-
 ?
2.3.1.150 acetyl-CoA + salutaridinol involved in morphine biosynthesis Papaver somniferum CoA + 7-O-acetylsalutaridinol
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.150 ? x * 52600, calculated from the deduced amino acid sequence Papaver somniferum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.150 47
-
-
 Papaver somniferum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.150 3.3
-
 nudaurine
-
 Papaver somniferum
2.3.1.150 4.4
-
 salutaridinol
-
 Papaver somniferum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.150 7 9
-
 Papaver somniferum