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Literature summary extracted from

  • Dai, Y.; Wensink, P.C.; Abeles, R.H.
    One protein, two enzymes (1999), J. Biol. Chem., 274, 1193-1195.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.53 expression in Escherichia coli Klebsiella pneumoniae
1.13.11.54 expression in Escherichia coli Klebsiella oxytoca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.53 500
-
 O2
-
 Klebsiella pneumoniae
1.13.11.53 500
-
 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one
-
 Klebsiella pneumoniae
1.13.11.54 210
-
 O2
-
 Klebsiella oxytoca
1.13.11.54 210
-
 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one
-
 Klebsiella oxytoca

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.53 Co2+ apoenzyme is catalytically inactive. Addition of Ni2+ or Co2+ yields activity. Production in intact Escherichia coli of E-2' depends on the availability of the Fe2+. Enzyme contains 1.1 Ni2+ per enzyme molecule Klebsiella pneumoniae
1.13.11.53 Ni2+ apoenzyme is catalytically inactive. Addition of Ni2+ or Co2+ yields activity. Production in intact Escherichia coli of E-2' depends on the availability of the Fe2+. Enzyme contains 1.1 Ni2+ per enzyme molecule Klebsiella pneumoniae
1.13.11.54 Fe2+ apoenzyme is catalytically inactive. Addition of Fe2+ yields activity. Production of the enzyme in intact Escherichia coli depends on the availability of the Fe2+. Enzyme contains 0.9 Fe2+ per enzyme molecule Klebsiella oxytoca

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.53 20250
-
 mass spectrometry Klebsiella pneumoniae
1.13.11.54 20240
-
 mass spectrometry Klebsiella oxytoca

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.53 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 Klebsiella pneumoniae enzyme of the methionine salvage pathway 3-(methylthio)propanoate + formate + CO
-
 ?
1.13.11.54 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 Klebsiella oxytoca enzyme of the methionine salvage pathway 4-(methylthio)-2-oxobutanoate + formate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.53 Klebsiella pneumoniae
-
-
-
1.13.11.54 Klebsiella oxytoca Q9ZFE7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.53 recombinant Klebsiella pneumoniae
1.13.11.54 recombinant Klebsiella oxytoca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.53 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 enzyme of the methionine salvage pathway Klebsiella pneumoniae 3-(methylthio)propanoate + formate + CO
-
 ?
1.13.11.53 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 Ni2+ or Co2+ bound to the enzyme protein. If Fe2+ is bound instead of Ni2+ reaction catalyzed by EC 1.13.11.54 occurs instead Klebsiella pneumoniae 3-(methylthio)propanoate + formate + CO
-
 ?
1.13.11.54 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 enzyme of the methionine salvage pathway Klebsiella oxytoca 4-(methylthio)-2-oxobutanoate + formate
-
 ?
1.13.11.54 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 Fe2+ bound to the enzyme protein. If Ni2+ or Co2+ is bound instead of Fe+, the reaction catalzed by EC 1.13.11.53 occurs instead Klebsiella oxytoca 4-(methylthio)-2-oxobutanoate + formate
-
 ?