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Literature summary extracted from

  • Hamai, A.; Hashimoto, N.; Mochizuki, H.; Kato, F.; Makiguchi, Y.; Horie, K.; Suzuki, S.
    Two distinct chondroitin sulfate ABC lyases. An endoeliminase yielding tetrasaccharides and an exoeliminase preferentially acting on oligosaccharides (1997), J. Biol. Chem., 272, 9123-9130.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.20 Cu2+ 1 mM, 80% inhibition Proteus vulgaris
4.2.2.20 Fe2+ 1 mM, 80% inhibition Proteus vulgaris
4.2.2.20 additional information not inhibitory: Ca2+, Mg2+, Ba2+, Mn2+ Proteus vulgaris
4.2.2.20 Ni2+ 1 mM, 80% inhibition Proteus vulgaris
4.2.2.20 Zn2+ 1 mM, complete inhibition Proteus vulgaris
4.2.2.21 additional information not inhibitory: Ni2+, Fe2+, Cu2+, Ca2+, Mg2+, Ba2+, Mn2+ Proteus vulgaris
4.2.2.21 Zn2+ 1 mM, complete inhibition Proteus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.20 0.066
-
 chondroitin-6-sulfate pH 8.0, 37°C Proteus vulgaris
4.2.2.21 0.08
-
 chondroitin-6-sulfate pH 8.0, 37°C Proteus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.20 Proteus vulgaris
-
 NCTC 4636
-
4.2.2.20 Proteus vulgaris
-
 induced by chondroitin sulfate, isoenzyme ChS ABC lyase II
-
4.2.2.21 Proteus vulgaris
-
 NCTC 4636
-
4.2.2.21 Proteus vulgaris
-
 NCTC 4636, induced by chondroitin sulfate, isoenzyme ChS ABC lyase II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.20
-
 Proteus vulgaris
4.2.2.21
-
 Proteus vulgaris

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.2.20 R1-[beta-D-GalNAc-1,4-L-iduronic acid]-R2 = R1-[beta-D-GalNAc] + [4-deoxy-L-idu-4-enuronic acid]-R2 related enzyme EC 4.2.2.21 Proteus vulgaris
a
4.2.2.21 [GlcAbeta(1-3)GalNAc(4S)beta(1-4)]3 = [GlcAbeta(1-3)GalNAc(4S)beta(1-4)]2 + 2-(acetylamino)-2-deoxy-3-O-alpha-L-threo-hex-4-enopyranuronosyl-4-O-sulfo-beta-D-galactopyranose related enzyme EC 4.2.2.20 Proteus vulgaris
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.20 310
-
 pH 8.0, 37°C Proteus vulgaris
4.2.2.21 34
-
 pH 8.0, 37°C Proteus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.20 chondroitin 32% of the rate with chondroitin-6-sulfate Proteus vulgaris ?
-
 ?
4.2.2.20 chondroitin 4-sulfate 121% of the rate with chondroitin-6-sulfate Proteus vulgaris ?
-
 ?
4.2.2.20 chondroitin 6-sulfate
-
 Proteus vulgaris unsaturated chondroitin 6-sulfate disaccharide
-
 ?
4.2.2.20 chondroitin sulfate D 70% of the rate with chondroitin-6-sulfate Proteus vulgaris ?
-
 ?
4.2.2.20 chondroitin sulfate E 23% of the rate with chondroitin-6-sulfate Proteus vulgaris ?
-
 ?
4.2.2.20 chondroitin sulfate proteoglycan 70% of the rate with chondroitin-6-sulfate Proteus vulgaris ?
-
 ?
4.2.2.20 dermatan sulfate 55% of the rate with chondroitin-6-sulfate Proteus vulgaris ?
-
 ?
4.2.2.20 hyaluronan 1% of the rate with chondroitin-6-sulfate Proteus vulgaris ?
-
 ?
4.2.2.20 additional information no substrate: keratan sulfate, heparin, heparan sulfate. Enzyme produces a wide range of differently sized oligosaccharides. After exhaustive degradation, end products are tetra- and disaccharides Proteus vulgaris ?
-
 ?
4.2.2.21 chondroitin 96% of the rate with chondroitin-6-sulfate Proteus vulgaris ?
-
 ?
4.2.2.21 chondroitin 4-sulfate 83% of the rate with chondroitin-6-sulfate Proteus vulgaris ?
-
 ?
4.2.2.21 chondroitin 6-sulfate
-
 Proteus vulgaris unsaturated chondroitin 6-sulfate disaccharide
-
 ?
4.2.2.21 chondroitin sulfate D 93% of the rate with chondroitin-6-sulfate Proteus vulgaris ?
-
 ?
4.2.2.21 chondroitin sulfate E 90% of the rate with chondroitin-6-sulfate Proteus vulgaris ?
-
 ?
4.2.2.21 chondroitin sulfate proteoglycan 67% of the rate with chondroitin-6-sulfate Proteus vulgaris ?
-
 ?
4.2.2.21 dermatan sulfate 77% of the rate with chondroitin-6-sulfate Proteus vulgaris ?
-
 ?
4.2.2.21 hyaluronan 17% of the rate with chondroitin-6-sulfate Proteus vulgaris ?
-
 ?
4.2.2.21 additional information no substrate: keratan sulfate, heparin, heparan sulfate. Activity of enzyme increases with decreasing of chain size from poly-, hexa-, to tetrasaccharide substrates Proteus vulgaris ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.2.20 ChS ABC lyase I
-
 Proteus vulgaris
4.2.2.20 EC 4.2.2.4 formerly Proteus vulgaris
4.2.2.21 ChS ABC lyase II
-
 Proteus vulgaris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.20 37
-
-
 Proteus vulgaris
4.2.2.21 40
-
-
 Proteus vulgaris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.20 40
-
 30 min, 47% loss of activity Proteus vulgaris
4.2.2.21 40
-
 30 min, 23% loss of activity Proteus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.20 8
-
-
 Proteus vulgaris
4.2.2.21 8
-
-
 Proteus vulgaris

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.2.20 Proteus vulgaris and 8.25, isoelectric focusing
-
 8
4.2.2.20 Proteus vulgaris and 8.0, isoelectric focusing
-
 8.25
4.2.2.21 Proteus vulgaris and 8.25, isoelectric focusing
-
 8
4.2.2.21 Proteus vulgaris and 8.0, isoelectric focusing
-
 8.25