EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.2.1.72 | C149A | no significant phosphorylating glyceraldehyde-3-phosphate dehydrogenase activity | Escherichia coli |
1.2.1.72 | C149G | no significant phosphorylating glyceraldehyde-3-phosphate dehydrogenase activity | Escherichia coli |
1.2.1.72 | C149V | no significant phosphorylating glyceraldehyde-3-phosphate dehydrogenase activity | Escherichia coli |
1.2.1.72 | C311A | mutation does not drastically change the phosphorylating glyceraldehyde-3-phosphate dehydrogenase activity. 5.7fold decrease in turnover number for the activity with D-erythrose 4-phosphate and NAD+ | Escherichia coli |
1.2.1.72 | C311Y | mutation does not drastically change the phosphorylating glyceraldehyde-3-phosphate dehydrogenase activity. 33.3fold decrease in turnover number for the activity with D-erythrose 4-phosphate and NAD+ | Escherichia coli |
1.2.1.72 | E32D | mutation does not drastically change the phosphorylating glyceraldehyde-3-phosphate dehydrogenase activity. 2.7fold decrease in turnover number for the activity with D-erythrose 4-phosphate and NAD+ | Escherichia coli |
1.2.1.72 | H176N | turnover number of phosphorylating glyceraldehyde-3-phosphate dehydrogenase activity is decreased by the factor 40. 95.2fold decrease in turnover number for the activity with D-erythrose 4-phosphate and NAD+ | Escherichia coli |
1.2.1.72 | M179T | mutation does not drastically change the phosphorylating glyceraldehyde-3-phosphate dehydrogenase activity. 5fold decrease in turnover number for the activity with D-erythrose 4-phosphate and NAD+ | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.72 | 0.24 | - |
D-erythrose 4-phosphate | 25°C, mutant enzyme H176N | Escherichia coli | |
1.2.1.72 | 0.5 | - |
NAD+ | 25°C, mutant enzyme H176N | Escherichia coli | |
1.2.1.72 | 0.51 | - |
D-erythrose 4-phosphate | 25°C, wild-type enzyme | Escherichia coli | |
1.2.1.72 | 0.7 | - |
D-erythrose 4-phosphate | 25°C, mutant enzyme C311Y | Escherichia coli | |
1.2.1.72 | 0.7 | - |
D-erythrose 4-phosphate | 25°C, mutant enzyme M179T | Escherichia coli | |
1.2.1.72 | 0.8 | - |
NAD+ | 25°C, wild-type enzyme | Escherichia coli | |
1.2.1.72 | 0.8 | - |
D-erythrose 4-phosphate | 25°C, mutant enzyme C311A | Escherichia coli | |
1.2.1.72 | 1.9 | - |
D-erythrose 4-phosphate | 25°C, mutant enzyme E32D | Escherichia coli | |
1.2.1.72 | 2.3 | - |
NAD+ | 25°C, mutant enzyme E32D | Escherichia coli | |
1.2.1.72 | 3.5 | - |
NAD+ | 25°C, mutant enzyme M179T | Escherichia coli | |
1.2.1.72 | 4 | - |
NAD+ | 25°C, mutant enzyme C311A | Escherichia coli | |
1.2.1.72 | 5 | - |
NAD+ | 25°C, mutant enzyme C311Y | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.72 | 37170 | - |
x * 37170, mass spectrometry | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.72 | Escherichia coli | - |
recombinant | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.72 | recombinant | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.1.72 | 14.7 | - |
- |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.72 | D-erythrose 4-phosphate + NAD+ | the chemical mechanism of erythrose 4-phosphate oxidation by gap-encoded protein proceeds through a two-step mechanism involving covalent intermediates with Cys149, with rates associated to the acylation and deacylation process of 280 per s and 20 per s, respectively. No isotopic solvent effect is observed, suggesting that the rate-limiting step is not hydrolysis | Escherichia coli | 4-phosphoerythronate + NADH | - |
? | |
1.2.1.72 | additional information | the enzyme also shows low phosphorylating glyceraldehyde-3-phosphate dehydrogenase activity | Escherichia coli | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.1.72 | ? | x * 37170, mass spectrometry | Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.72 | GapB-encoded protein | - |
Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.72 | 0.21 | - |
NAD+ | 25°C, mutant enzyme H176N | Escherichia coli | |
1.2.1.72 | 0.21 | - |
D-erythrose 4-phosphate | 25°C, mutant enzyme H176N | Escherichia coli | |
1.2.1.72 | 0.6 | - |
NAD+ | 25°C, mutant enzyme C311Y | Escherichia coli | |
1.2.1.72 | 0.6 | - |
D-erythrose 4-phosphate | 25°C, mutant enzyme C311Y | Escherichia coli | |
1.2.1.72 | 3.5 | - |
NAD+ | 25°C, mutant enzyme C311A | Escherichia coli | |
1.2.1.72 | 3.5 | - |
D-erythrose 4-phosphate | 25°C, mutant enzyme C311A | Escherichia coli | |
1.2.1.72 | 4 | - |
NAD+ | 25°C, mutant enzyme M179T | Escherichia coli | |
1.2.1.72 | 4 | - |
D-erythrose 4-phosphate | 25°C, mutant enzyme M179T | Escherichia coli | |
1.2.1.72 | 7.4 | - |
NAD+ | 25°C, mutant enzyme E32D | Escherichia coli | |
1.2.1.72 | 7.4 | - |
D-erythrose 4-phosphate | 25°C, mutant enzyme E32D | Escherichia coli | |
1.2.1.72 | 20 | - |
NAD+ | 25°C, wild-type enzyme | Escherichia coli | |
1.2.1.72 | 20 | - |
D-erythrose 4-phosphate | 25°C, wild-type enzyme | Escherichia coli |