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Literature summary extracted from

  • Culver, G.M.; McCraith, S.M.; Consaul, S.A.; Stanford, D.R.; Phizicky, E.M.
    A 2'-phosphotransferase implicated in tRNA splicing is essential in Saccharomyces cerevisiae (1997), J. Biol. Chem., 272, 13203-13210.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.160 expression in Escherichia coli strain RZ510 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.160 30000
-
 gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.160 Escherichia coli
-
-
-
2.7.1.160 Homo sapiens
-
-
-
2.7.1.160 Mus musculus
-
-
-
2.7.1.160 Saccharomyces cerevisiae
-
-
-
2.7.1.160 Saccharomyces cerevisiae Q12272
-
-
2.7.1.160 Schizosaccharomyces pombe
-
 clone c2c4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.160 purified 28000fold by Orange A Sepharose column, 4fold less pure by streamlined purification procedure Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.7.1.160 -20°C, Tris-buffer, pH 7.5, 50% (m/v) glycerol, 3 months Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.160 2'-phospho-[ligated tRNA] + NAD+
-
 Saccharomyces cerevisiae mature tRNA + ADP ribose-1'',2''-phosphate + nicotinamide + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.160 Tpt1
-
 Saccharomyces cerevisiae