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Literature summary extracted from

  • Abdullah, M.; Hughes, P.J.; Craxton, A.; Gigg, R.; Desai, T.; Marecek, J.F.; Prestwich, G.D.; Shears, S.B.
    Purification and characterization of inositol-1,3,4-trisphosphate 5/6-kinase from rat liver using an inositol hexakisphosphate affinity column (1992), J. Biol. Chem., 267, 22340-22345.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.159 additional information enzyme activity is unaffected by protein kinase C, protein kinase A, and Ca2+/calmodulin Rattus norvegicus

General Stability

EC Number General Stability Organism
2.7.1.159 addition of 1 mM ATP at pH 6.1 stabilizes the enzyme during purification Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.159 1D-myo-inositol-1,3,4,5-tetrakisphosphate competitive Rattus norvegicus
2.7.1.159 1D-myo-inositol-1,3,4,6-tetrakisphosphate competitive Rattus norvegicus
2.7.1.159 additional information enzyme activity is unaffected by protein kinase C, protein kinase A, and Ca2+/calmodulin Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.159 additional information
-
 additional information kinetics Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.159 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.159 Mg2+
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.159 36000
-
 x * 36000, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.159 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.159 26171fold from liver by polyethylene glycol precipitation, ion exchange chromatography and heparin and inositol hexakisphosphate affinity chromatograpies Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.159 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.159 1780
-
 purified enzyme, determination of 5-kinase and 6-kinase activities Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.159 ATP + 1D-myo-inositol-1,3,4-trisphosphate 5fold lower activity compared to 1D-myo-inositol-1,3,4,6-tetrakisphosphate formation Rattus norvegicus ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.159 ATP + 1D-myo-inositol-1,3,4-trisphosphate 5fold higher activity compared to 1D-myo-inositol-1,3,4,5-tetrakisphosphate formation Rattus norvegicus ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.159 ? x * 36000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.159 inositol-1,3,4-trisphosphate kinase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.159 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.159 7.2
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.159 ATP
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.159 0.002 0.003 1D-myo-inositol-1,3,4,6-tetrakisphosphate pH 7.2, 37°C, versus 1D-myo-inositol-1,3,4-trisphosphate Rattus norvegicus
2.7.1.159 0.003 0.004 1D-myo-inositol-1,3,4,5-tetrakisphosphate pH 7.2, 37°C, versus 1D-myo-inositol-1,3,4-trisphosphate Rattus norvegicus