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Literature summary extracted from

  • Adewale, I.O.; Afolayan, A.
    Purification and catalytic properties of glutathione transferase from the hepatopancreas of crayfish Macrobrachium vollenhovenii (herklots) (2004), J. Biochem. Mol. Toxicol., 18, 332-344.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.5.1.18 low guanidine-HCl concentrations, less than 0.5 M, denature the enzyme, complete renaturation is possible. At higher concentrations of the denaturant, 0.5-4 M, complete renaturation is not achieved. The extent of renaturation is a function of protein concentration Macrobrachium vollenhovenii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 1-chloro-2,3-dinitrobenzene
-
 Macrobrachium vollenhovenii
2.5.1.18 1-chloro-2,4-dinitrobenzene
-
 Macrobrachium vollenhovenii
2.5.1.18 Cibacron blue IC50: 10 nM Macrobrachium vollenhovenii
2.5.1.18 GSH
-
 Macrobrachium vollenhovenii
2.5.1.18 Hematin IC50: 0.005 mM Macrobrachium vollenhovenii
2.5.1.18 NEM IC50: 33 mM Macrobrachium vollenhovenii
2.5.1.18 S-hexylglutathione IC50: 0.34 mM Macrobrachium vollenhovenii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 1.31
-
 GSH
-
 Macrobrachium vollenhovenii
2.5.1.18 2.03
-
 1-chloro-2,4-dinitrobenzene
-
 Macrobrachium vollenhovenii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.18 24100
-
 2 * 24100, SDS-PAGE Macrobrachium vollenhovenii
2.5.1.18 46000
-
 gel filtration Macrobrachium vollenhovenii

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Macrobrachium vollenhovenii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18
-
 Macrobrachium vollenhovenii

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.5.1.18 low guanidine-HCl concentrations, less than 0.5 M, denature the enzyme, complete renaturation is possible. At higher concentrations of the denaturant, 0.5-4 M, complete renaturation is not achieved. The extent of renaturation is a function of protein concentration Macrobrachium vollenhovenii

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 12.36
-
-
 Macrobrachium vollenhovenii

Storage Stability

EC Number Storage Stability Organism
2.5.1.18 -20°C, 10% glycerol, stable for 4 months Macrobrachium vollenhovenii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 1-chloro-2,4-dinitrobenzene + GSH
-
 Macrobrachium vollenhovenii S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
2.5.1.18 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole + GSH
-
 Macrobrachium vollenhovenii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 dimer 2 * 24100, SDS-PAGE Macrobrachium vollenhovenii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.18 20.3
-
 GSH
-
 Macrobrachium vollenhovenii
2.5.1.18 20.3
-
 1-chloro-2,4-dinitrobenzene
-
 Macrobrachium vollenhovenii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.18 1.09
-
 1-chloro-2,4-dinitrobenzene
-
 Macrobrachium vollenhovenii
2.5.1.18 1.13
-
 GSH
-
 Macrobrachium vollenhovenii

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.5.1.18 0.00001
-
 IC50: 10 nM Macrobrachium vollenhovenii Cibacron blue
2.5.1.18 0.005
-
 IC50: 0.005 mM Macrobrachium vollenhovenii Hematin
2.5.1.18 0.34
-
 IC50: 0.34 mM Macrobrachium vollenhovenii S-hexylglutathione
2.5.1.18 33
-
 IC50: 33 mM Macrobrachium vollenhovenii NEM