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Literature summary extracted from

  • Miyashiro, M.; Furuya, S.; Sugita, T.
    Development of a sensitive separation and quantification method for sialyl Lewis X and Lewis X involving anion-exchange chromatography: biochemical characterization of alpha1-3 fucosyltransferase-VII (2004), J. Biochem., 136, 723-731.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.152 FucT-VII: DNA and amino acid sequence determination and analysis, expression of the catalytic domain of FucT-VII fused to protein A from Staphylococcus aureus in Spodoptera frugiperda Sf21 insect cells via the baculovirus infection system Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.152 GDP strong inhibition of FucT-VII Homo sapiens
2.4.1.152 additional information no inhibition of FucT-VII by NEM Homo sapiens
2.4.1.152 NEM complete inhibition of FucT-VI at 5 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.152 0.021
-
 GDP-beta-fucose pH 6.5, 30°C, recombinant FucT-VII and commercial FucT-VI Homo sapiens
2.4.1.152 0.3
-
 N-acetyllactosamine pH 6.5, 30°C, commercial FucT-VI Homo sapiens
2.4.1.152 7.8
-
 alpha2,3-sialyl-N-acetyllactosamine pH 6.5, 30°C, recombinant FucT-VII Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.152 Golgi membrane the enzyme possesses a cytoplasmic domain, a transmembrane domain, and a Golgi lumenal catalytic domain Homo sapiens 139
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.152 Mn2+ at 10 mM MnCl2 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.152 39000
-
 x * 39000, wild-type enzyme, SDS-PAGE, x * 44000-46000, recombinant FucT-VII catalytic domain-protein A fusion protein, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.152 GDP-beta-fucose + alpha2,3-sialyl N-acetyllactosaminyl-R Homo sapiens FucT-VII and FucT-IV GDP + alpha1,3-fucosyl-alpha2,3-sialyl-N-acetyllactosaminyl-R
-
 ?
2.4.1.152 additional information Homo sapiens FucT-VI is involved in the biosynthesis of nonsialylated Lewis X, i.e. Lex, antigen, FucT VII is essential for biosynthesis of the carbohydrate antigen sialyl Lewis X, i.e. sLex, in leukocytes, FucT VII and FucT IV are essential for biosynthesis of selectin binding ligands in T-cells ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.152 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.152 glycoprotein the wild-type contains 2 N-glycosylation sites Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.152 recombinant FucT-VII catalytic domain-protein A fusion protein from Sf21 insect cells by IgG affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.152 commercial preparation FucT-VI Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.152 additional information
-
 assay development utilizing anion exchange chromatography, overview Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.152 GDP-beta-fucose + alpha2,3-sialyl N-acetyllactosamine FucT-VII and FucT-IV Homo sapiens GDP + alpha1,3-fucosyl-alpha2,3-sialyl-N-acetyllactosamine
-
 ?
2.4.1.152 GDP-beta-fucose + alpha2,3-sialyl N-acetyllactosaminyl-R FucT-VII and FucT-IV Homo sapiens GDP + alpha1,3-fucosyl-alpha2,3-sialyl-N-acetyllactosaminyl-R
-
 ?
2.4.1.152 GDP-beta-fucose + N-acetyllactosamine FucT-VI Homo sapiens GDP + alpha1,3-fucosyl-N-acetyllactosamine
-
 ?
2.4.1.152 additional information FucT-VI is involved in the biosynthesis of nonsialylated Lewis X, i.e. Lex, antigen, FucT VII is essential for biosynthesis of the carbohydrate antigen sialyl Lewis X, i.e. sLex, in leukocytes, FucT VII and FucT IV are essential for biosynthesis of selectin binding ligands in T-cells Homo sapiens ?
-
 ?
2.4.1.152 additional information assay development utilizing anion exchange chromatography Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.152 ? x * 39000, wild-type enzyme, SDS-PAGE, x * 44000-46000, recombinant FucT-VII catalytic domain-protein A fusion protein, SDS-PAGE Homo sapiens
2.4.1.152 More the enzyme possesses a cytoplasmic domain, a transmembrane domain, and a large Golgi lumenal catalytic domain Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.4.1.152 alpha1-3 FucT
-
 Homo sapiens
2.4.1.152 FucT-VI
-
 Homo sapiens
2.4.1.152 FucT-VII
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.152 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.152 5.5 6.5 recombinant FucT-VII Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.152 0.0045
-
 GDP pH 6.5, 30°C, recombinant FucT-VII Homo sapiens