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Literature summary extracted from

  • Cai, L.; Hu, C.; Shen, S.; Wang, W.; Huang, W.
    Characterization of bacteriophage T3 DNA ligase (2004), J. Biochem., 135, 397-403.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.1 expression in Escherichia coli, polyhistidine-tagged enzyme Enterobacteria phage T3

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.1 ATP 2 mM 50% inhibition Enterobacteria phage T3

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5.1.1 0.0144
-
 ATP ligation of cohesive DNA Enterobacteria phage T3

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.1 Ca2+ 5 mM, increases activity 9fold Enterobacteria phage T3
6.5.1.1 Mg2+ 5 mM, increases activity 9fold Enterobacteria phage T3
6.5.1.1 Mn2+ 5 mM, increases activity 7fold Enterobacteria phage T3
6.5.1.1 NaCl promotes enzyme activity, optimal concentration: 300 mM Enterobacteria phage T3

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Enterobacteria phage T3
-
 AMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?
6.5.1.1 dATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Enterobacteria phage T3
-
 dAMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Enterobacteria phage T3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.1 recombinant polyhistidine-tagged enzyme Enterobacteria phage T3

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Enterobacteria phage T3 AMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?
6.5.1.1 dATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Enterobacteria phage T3 dAMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.5.1.1 8
-
-
 Enterobacteria phage T3

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5.1.1 8
-
-
 Enterobacteria phage T3

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.5.1.1 6.5 8.5 pH 6.5: about 80% of maximal activity, pH 8.5: about 85% of maximal activity Enterobacteria phage T3

pI Value

EC Number Organism Comment pI Value Maximum pI Value
6.5.1.1 Enterobacteria phage T3 calculation from amino acid sequence
-
 5.14