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Literature summary extracted from

  • Cheng, W.C.; Chang, Y.N.; Wang, W.C.
    Structural basis for shikimate-binding specificity of Helicobacter pylori shikimate kinase (2005), J. Bacteriol., 187, 8156-8163.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.71 expressed in Escherichia coli JM109 cells Helicobacter pylori

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.71 hanging drop vapour diffusion method using containing 0.2 M lithium sulfate, 30% (wt/vol) PEG 8000, and 0.1 M sodium acetate buffer (pH 6.5), at 20°C Helicobacter pylori
2.7.1.71 hanging-drop vapor diffusion method. 1.8 A crystal structure of shikimate kinase. The crystal structure shows a three-layer alpha/beta fold consisting of a central sheet of five parallel beta-strands flanked by seven alpha-helices. An HpSK-shikimate-PO4 complex is also determined and refined to 2.3 A, revealing induced-fit movement from an open to a closed form on substrate binding Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.71 0.06
-
 shikimate in 100 mM Tris-HCl–KOH buffer, pH 7.5, 50 mM KCl, 5 mM MgCl2, 1.6 mM shikimic acid, 2.5 mM ATP, 1 mM phosphoenolpyruvate, 0.1 mM NADH, 2.5 units of pyruvate kinase/ml, and 2.7 units of lactate dehydrogenase/ml, at 25°C Helicobacter pylori
2.7.1.71 0.101
-
 ATP in 100 mM Tris-HCl-KOH buffer, pH 7.5, 50 mM KCl, 5 mM MgCl2, 1.6 mM shikimic acid, 2.5 mM ATP, 1 mM phosphoenolpyruvate, 0.1 mM NADH, 2.5 units of pyruvate kinase/ml, and 2.7 units of lactate dehydrogenase/ml, at 25°C Helicobacter pylori

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.71 Mg2+ required for activity Helicobacter pylori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.71 20000
-
 SDS-PAGE Helicobacter pylori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.71 ATP + shikimate Helicobacter pylori fifth step in the shikimate pathway ADP + shikimate 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.71 Helicobacter pylori P56073
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.71 immobilized-nickel ion chromatography and Superdex-75 gel filtration Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.71 ATP + shikimate
-
 Helicobacter pylori ADP + shikimate 3-phosphate
-
 ?
2.7.1.71 ATP + shikimate fifth step in the shikimate pathway Helicobacter pylori ADP + shikimate 3-phosphate
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.71 ATP
-
 Helicobacter pylori