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Literature summary extracted from

  • Keppetipola, N.; Shuman, S.
    Characterization of a thermophilic ATP-dependent DNA ligase from the euryarchaeon Pyrococcus horikoshii (2005), J. Bacteriol., 187, 6902-6908.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.1 ADP inhibits catalysis of nick-sealing by the ligase-AMP intermediate Pyrococcus horikoshii
6.5.1.1 Ca2+ partially inhibitory, ligation activity in presence of Mg2+ Pyrococcus horikoshii
6.5.1.1 Cd2+ 5 mM, abolishes ligation reaction in presence of 5 mM Mg2+ Pyrococcus horikoshii
6.5.1.1 Co2+ 5 mM, abolishes ligation reaction in presence of 5 mM Mg2+ Pyrococcus horikoshii
6.5.1.1 CTP 2 mM, 18% inhibition of single-turnover ligation Pyrococcus horikoshii
6.5.1.1 Cu2+ partially inhibitory, ligation activity in presence of Mg2+ Pyrococcus horikoshii
6.5.1.1 GTP 2 mM, 38% inhibition of single-turnover ligation Pyrococcus horikoshii
6.5.1.1 Mn2+ 5 mM, abolishes ligation reaction in presence of 5 mM Mg2+ Pyrococcus horikoshii
6.5.1.1 Ni2+ 5 mM, abolishes ligation reaction in presence of 5 mM Mg2+ Pyrococcus horikoshii
6.5.1.1 UTP 2 mM, 7% inhibition of single-turnover ligation Pyrococcus horikoshii
6.5.1.1 Zn2+ 5 mM, abolishes ligation reaction in presence of 5 mM Mg2+ Pyrococcus horikoshii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.1 Ca2+ 1 mM, supports autoadenylation reaction. Does not support nick-joining activity at 2-5 mM Pyrococcus horikoshii
6.5.1.1 Cd2+ 1 mM, supports autoadenylation reaction. Does not support nick-joining activity at 2-5 mM Pyrococcus horikoshii
6.5.1.1 Co2+ 1 mM, supports autoadenylation reaction. Does not support nick-joining activity at 2-5 mM Pyrococcus horikoshii
6.5.1.1 Cu2+ 1 mM, supports autoadenylation reaction. Does not support nick-joining activity at 2-5 mM Pyrococcus horikoshii
6.5.1.1 Mg2+ 1 mM, supports autoadenylation reaction. Required for nick-joining activity at 2-5 mM Pyrococcus horikoshii
6.5.1.1 Mn2+ 1 mM, supports autoadenylation reaction. Supports nick-joining activity to a much lesser extent than Mg2+ Pyrococcus horikoshii
6.5.1.1 Ni2+ 1 mM, supports autoadenylation reaction. Does not support nick-joining activity at 2-5 mM Pyrococcus horikoshii
6.5.1.1 Zn2+ 1 mM, supports autoadenylation reaction. Does not support nick-joining activity at 2-5 mM Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Pyrococcus horikoshii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.1
-
 Pyrococcus horikoshii

Subunits

EC Number Subunits Comment Organism
6.5.1.1 monomer
-
 Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
6.5.1.1 ATP-dependent DNA ligase
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.5.1.1 80
-
 ligation reaction Pyrococcus horikoshii
6.5.1.1 90
-
 adenylyltransferase activity Pyrococcus horikoshii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.5.1.1 60 90 60°C: about 35% of maximal activity, 90°C: about 90% of maximal activity, ligation activity Pyrococcus horikoshii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5.1.1 7
-
 adenylyltransferase activity Pyrococcus horikoshii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.5.1.1 5 8.5 pH 5.0: about 65% of maximal activity, pH 8.5: about 45% of maximal activity, adenylyltransferase activity Pyrococcus horikoshii