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Literature summary extracted from

  • Marcus, Y.; Altman-Gueta, H.; Finkler, A.; Gurevitz, M.
    Mutagenesis at two distinct phosphate-binding sites unravels their differential roles in regulation of Rubisco activation and catalysis (2005), J. Bacteriol., 187, 4222-4228.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.39 H310A turnover number for D-ribulose 1,5-bisphosphate is nearly identical to wild-type value, 1.3fold increase in KM-value for D-ribulose 1,5-bisphosphate Synechocystis sp.
4.1.1.39 H310D 2.9fold decrease in turnover number for D-ribulose 1,5-bisphosphate, 2.8fold increase in KM-value for D-ribulose 1,5-bisphosphate Synechocystis sp.
4.1.1.39 H327Q 5.9fold decrease in turnover number for D-ribulose 1,5-bisphosphate, 3.5fold increase in KM-value for D-ribulose 1,5-bisphosphate, 8.7fold decrease in Ki-value for phosphate Synechocystis sp.
4.1.1.39 H398A 12.7fold decrease in turnover number for D-ribulose 1,5-bisphosphate, 1.9fold increase in KM-value for D-ribulose 1,5-bisphosphate Synechocystis sp.
4.1.1.39 K305A 3.1fold decrease in turnover number for D-ribulose 1,5-bisphosphate, 1.4fold increase in KM-value for D-ribulose 1,5-bisphosphate Synechocystis sp.
4.1.1.39 K305E 2.9fold decrease in turnover number for D-ribulose 1,5-bisphosphate, 1.7fold increase in KM-value for D-ribulose 1,5-bisphosphate Synechocystis sp.
4.1.1.39 R134A 3.1fold decrease in turnover number for D-ribulose 1,5-bisphosphate, 1.5fold increase in KM-value for D-ribulose 1,5-bisphosphate Synechocystis sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.39 phosphate
-
 Synechocystis sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.39 0.14
-
 D-ribulose 1,5-bisphosphate wild-type enzyme Synechocystis sp.
4.1.1.39 0.161
-
 CO2 mutant enzyme R134A Synechocystis sp.
4.1.1.39 0.179
-
 D-ribulose 1,5-bisphosphate mutant enzyme H310A Synechocystis sp.
4.1.1.39 0.181
-
 CO2 wild-type enzyme Synechocystis sp.
4.1.1.39 0.185
-
 CO2 mutant enzyme K305A Synechocystis sp.
4.1.1.39 0.19
-
 CO2 mutant enzyme K305E Synechocystis sp.
4.1.1.39 0.199
-
 D-ribulose 1,5-bisphosphate mutant enzyme K305A Synechocystis sp.
4.1.1.39 0.212
-
 D-ribulose 1,5-bisphosphate mutant enzyme R134A Synechocystis sp.
4.1.1.39 0.242
-
 D-ribulose 1,5-bisphosphate mutant enzyme K305E Synechocystis sp.
4.1.1.39 0.267
-
 D-ribulose 1,5-bisphosphate mutant enzyme H298A Synechocystis sp.
4.1.1.39 0.387
-
 D-ribulose 1,5-bisphosphate mutant enzyme H310D Synechocystis sp.
4.1.1.39 0.49
-
 D-ribulose 1,5-bisphosphate mutant enzyme H327Q Synechocystis sp.

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.39 Synechocystis sp.
-
 strain PCC 6803
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.39 D-ribulose 1,5-bisphosphate + CO2 + H2O
-
 Synechocystis sp. 3-phospho-D-glycerate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.39 0.72
-
 D-ribulose 1,5-bisphosphate mutant enzyme H298A Synechocystis sp.
4.1.1.39 1.53
-
 D-ribulose 1,5-bisphosphate mutant enzyme H327Q Synechocystis sp.
4.1.1.39 2.95
-
 D-ribulose 1,5-bisphosphate mutant enzyme K305A Synechocystis sp.
4.1.1.39 2.97
-
 D-ribulose 1,5-bisphosphate mutant enzyme R134A Synechocystis sp.
4.1.1.39 3.08
-
 D-ribulose 1,5-bisphosphate mutant enzyme H310D Synechocystis sp.
4.1.1.39 3.08
-
 D-ribulose 1,5-bisphosphate mutant enzyme K305E Synechocystis sp.
4.1.1.39 9
-
 D-ribulose 1,5-bisphosphate mutant enzyme H310A Synechocystis sp.
4.1.1.39 9.08
-
 D-ribulose 1,5-bisphosphate wild-type enzyme Synechocystis sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.39 0.67
-
 phosphate mutant enzyme H327Q Synechocystis sp.
4.1.1.39 5.7
-
 phosphate mutant enzyme K305E Synechocystis sp.
4.1.1.39 5.8
-
 phosphate wild-type enzyme Synechocystis sp.
4.1.1.39 6
-
 phosphate mutant enzyme R134A Synechocystis sp.
4.1.1.39 6.3
-
 phosphate mutant enzyme H298A Synechocystis sp.