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Literature summary extracted from

  • Manukhov, I.V.; Mamaeva, D.V.; Rastorguev, S.M.; Faleev, N.G.; Morozova, E.A.; Demidkina, T.V.; Zavilgelsky, G.B.
    A gene encoding L-methionine gamma-lyase is present in Enterobacteriaceae family genomes: identification and characterization of Citrobacter freundii L-methionine gamma-lyase (2005), J. Bacteriol., 187, 3889-3893.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.11 expression in Escherichia coli Citrobacter freundii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.11 0.16
-
 L-cysteine recombinant enzyme Citrobacter freundii
4.4.1.11 0.17
-
 S-ethyl-L-cysteine recombinant enzyme Citrobacter freundii
4.4.1.11 0.18
-
 L-cysteine wild-type Citrobacter freundii
4.4.1.11 0.18
-
 S-Benzyl-L-cysteine recombinant enzyme Citrobacter freundii
4.4.1.11 0.19
-
 S-Benzyl-L-cysteine wild-type Citrobacter freundii
4.4.1.11 0.49
-
 S-ethyl-L-cysteine wild-type Citrobacter freundii
4.4.1.11 0.54
-
 L-ethionine recombinant enzyme Citrobacter freundii
4.4.1.11 0.56
-
 L-ethionine wild-type Citrobacter freundii
4.4.1.11 0.61
-
 S-methyl-L-cysteine wild-type Citrobacter freundii
4.4.1.11 0.7
-
 L-methionine wild-type Citrobacter freundii
4.4.1.11 0.7
-
 L-methionine recombinant enzyme Citrobacter freundii
4.4.1.11 0.71
-
 S-methyl-L-cysteine recombinant enzyme Citrobacter freundii
4.4.1.11 0.97
-
 DL-homocysteine recombinant enzyme Citrobacter freundii
4.4.1.11 1.1
-
 DL-homocysteine wild-type Citrobacter freundii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.11 43000 45000 SDS-PAGE Citrobacter freundii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.11 L-methionine + H2O Citrobacter freundii
-
 methanethiol + NH3 + 2-oxobutanoate
-
 ?
4.4.1.11 L-methionine + H2O Citrobacter freundii ATCC 29063
-
 methanethiol + NH3 + 2-oxobutanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.11 Citrobacter freundii Q84AR1 ATCC29063
-
4.4.1.11 Citrobacter freundii ATCC 29063 Q84AR1 ATCC29063
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.11
-
 Citrobacter freundii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.11 10
-
 purified enzyme Citrobacter freundii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.11 DL-homocysteine + H2O
-
 Citrobacter freundii hydrogen sulfide + NH3 + 2-oxobutanoate
-
 ?
4.4.1.11 DL-homocysteine + H2O
-
 Citrobacter freundii ATCC 29063 hydrogen sulfide + NH3 + 2-oxobutanoate
-
 ?
4.4.1.11 L-cysteine + H2O
-
 Citrobacter freundii sulfide + NH3 + pyruvate
-
 ?
4.4.1.11 L-cysteine + H2O
-
 Citrobacter freundii ATCC 29063 sulfide + NH3 + pyruvate
-
 ?
4.4.1.11 L-ethionine + H2O
-
 Citrobacter freundii ethanethiol + NH3 + 2-oxobutanoate
-
 ?
4.4.1.11 L-ethionine + H2O
-
 Citrobacter freundii ATCC 29063 ethanethiol + NH3 + 2-oxobutanoate
-
 ?
4.4.1.11 L-methionine + H2O
-
 Citrobacter freundii methanethiol + NH3 + 2-oxobutanoate
-
 ?
4.4.1.11 L-methionine + H2O
-
 Citrobacter freundii ATCC 29063 methanethiol + NH3 + 2-oxobutanoate
-
 ?
4.4.1.11 S-benzyl-L-cysteine + H2O
-
 Citrobacter freundii thiobenzyl alcohol + NH3 + 2-oxopropanoate
-
 ?
4.4.1.11 S-benzyl-L-cysteine + H2O
-
 Citrobacter freundii ATCC 29063 thiobenzyl alcohol + NH3 + 2-oxopropanoate
-
 ?
4.4.1.11 S-ethyl-L-cysteine + H2O
-
 Citrobacter freundii ethanethiol + NH3 + 2-oxopropanoate
-
 ?
4.4.1.11 S-methyl-L-cysteine + H2O
-
 Citrobacter freundii methanethiol + NH3 + 2-oxopropanoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.4.1.11 L-methionine gamma-lyase
-
 Citrobacter freundii
4.4.1.11 MGL
-
 Citrobacter freundii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4.1.11 2.24
-
 L-cysteine wild-type Citrobacter freundii
4.4.1.11 2.33
-
 L-cysteine recombinant enzyme Citrobacter freundii
4.4.1.11 4.6
-
 S-methyl-L-cysteine recombinant enzyme Citrobacter freundii
4.4.1.11 5.03
-
 S-ethyl-L-cysteine recombinant enzyme Citrobacter freundii
4.4.1.11 5.1
-
 DL-homocysteine wild-type Citrobacter freundii
4.4.1.11 5.97
-
 S-methyl-L-cysteine wild-type Citrobacter freundii
4.4.1.11 6.2
-
 L-ethionine wild-type Citrobacter freundii
4.4.1.11 6.2
-
 L-methionine recombinant enzyme Citrobacter freundii
4.4.1.11 6.5
-
 L-methionine wild-type Citrobacter freundii
4.4.1.11 6.78
-
 L-ethionine recombinant enzyme Citrobacter freundii
4.4.1.11 6.8
-
 S-ethyl-L-cysteine wild-type Citrobacter freundii
4.4.1.11 8.16
-
 S-Benzyl-L-cysteine recombinant enzyme Citrobacter freundii
4.4.1.11 8.51
-
 DL-homocysteine recombinant enzyme Citrobacter freundii
4.4.1.11 10.9
-
 S-Benzyl-L-cysteine wild-type Citrobacter freundii