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Literature summary extracted from

  • Caescu, C.I.; Vidal, O.; Krzewinski, F.; Artenie, V.; Bouquelet, S.
    Bifidobacterium longum requires a fructokinase (Frk; ATP:D-fructose 6-phosphotransferase, EC 2.7.1.4) for fructose catabolism (2004), J. Bacteriol., 186, 6515-6525.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.4 ATP inhibitory above 12 mM Bifidobacterium longum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.4 0.739
-
 D-fructose pH 6.5, 50°C Bifidobacterium longum
2.7.1.4 0.756
-
 ATP
-
 Bifidobacterium longum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.4 32500
-
 x * 32500, calculated, x * 35000, SDS-PAGE Bifidobacterium longum
2.7.1.4 35000
-
 x * 32500, calculated, x * 35000, SDS-PAGE Bifidobacterium longum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.4 Bifidobacterium longum
-
 induction by D-fructose, repression by D-glucose, expressed in Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.4 recombinant protein Bifidobacterium longum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.4 0.84
-
 substrate D-fructose, pH 6.5, 50°C Bifidobacterium longum
2.7.1.4 1.8
-
 substrate ATP, pH 6.5, 50°C Bifidobacterium longum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.4 ATP + D-fructose highly specific for D-fructose Bifidobacterium longum ADP + D-fructose 6-phosphate
-
 ?
2.7.1.4 CTP + D-fructose 6% of activity with ATP Bifidobacterium longum CDP + D-fructose 6-phosphate
-
 ?
2.7.1.4 GTP + D-fructose 23% of activity with ATP Bifidobacterium longum GDP + D-fructose 6-phosphate
-
 ?
2.7.1.4 ITP + D-fructose 78% of activity with ATP Bifidobacterium longum IDP + D-fructose 6-phosphate
-
 ?
2.7.1.4 TTP + D-fructose 0.18% of activity with ATP Bifidobacterium longum TDP + D-fructose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.4 ? x * 32500, calculated, x * 35000, SDS-PAGE Bifidobacterium longum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.4 50
-
-
 Bifidobacterium longum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.4 6 40 gradual decrease of activtiy in this range Bifidobacterium longum
2.7.1.4 37 39 15 min, 70% residual activity Bifidobacterium longum
2.7.1.4 60
-
 15 min, complete loss of activity Bifidobacterium longum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.4 6
-
-
 Bifidobacterium longum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.4 5 8 marked decrease in activity below and above Bifidobacterium longum