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Literature summary extracted from

  • Voynova, N.E.; Rios, S.E.; Miziorko, H.M.
    Staphylococcus aureus mevalonate kinase: isolation and characterization of an enzyme of the isoprenoid biosynthetic pathway (2004), J. Bacteriol., 186, 61-67.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.36
-
 Homo sapiens
2.7.1.36
-
 Rattus norvegicus
2.7.1.36 expressed in Escherichia coli BL21(DE3) Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.36 5-phosphomevalonate product inhibition, non-competitive with ATP and mevalonate Staphylococcus aureus
2.7.1.36 farnesyl diphosphate feedback inhibition, competitive with ATP Homo sapiens
2.7.1.36 farnesyl diphosphate feedback inhibition, competitive with ATP Rattus norvegicus
2.7.1.36 farnesyl diphosphate feedback inhibition, competitive with ATP Staphylococcus aureus
2.7.1.36 farnesyl thiodiphosphate feedback inhibition, competitive with ATP Homo sapiens
2.7.1.36 farnesyl thiodiphosphate feedback inhibition, competitive with ATP Rattus norvegicus
2.7.1.36 farnesyl thiodiphosphate feedback inhibition, competitive with ATP Staphylococcus aureus
2.7.1.36 mevalonate substrate inhibition at millimolar concentrations, 50% inhibition at 2.88 mM Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.36 0.041
-
 R,S-mevalonate pH 7.5, 30°C Staphylococcus aureus
2.7.1.36 0.339
-
 ATP pH 7.5, 30°C Staphylococcus aureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.36 cytosol
-
 Staphylococcus aureus 5829
-
2.7.1.36 cytosol
-
 Homo sapiens 5829
-
2.7.1.36 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.36 Mg2+ required Staphylococcus aureus
2.7.1.36 Mn2+ 25% of the activity with Mg2+ Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.36 33000
-
 1 * 33000, SDS-PAGE and gel filtration Staphylococcus aureus
2.7.1.36 34500
-
 gel filtration Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.36 ATP + (R)-mevalonate Rattus norvegicus involved in cholesterol synthesis ADP + (R)-5-phosphomevalonate
-
 ?
2.7.1.36 ATP + (R)-mevalonate Homo sapiens involved in cholesterol synthesis, decreased enzyme activity involved in mevalonic aciduria and hyperimmunoglobulin D syndrome ADP + (R)-5-phosphomevalonate
-
 ?
2.7.1.36 ATP + (R)-mevalonate Staphylococcus aureus involved in isopentenyl diphosphate synthesis ADP + (R)-5-phosphomevalonate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.36 Homo sapiens
-
-
-
2.7.1.36 Rattus norvegicus
-
-
-
2.7.1.36 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.36 recombinant enzyme Staphylococcus aureus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.36 12.4
-
 purified enzyme, pH 7.5, 30°C Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.36 ATP + (R)-mevalonate
-
 Staphylococcus aureus ADP + (R)-5-phosphomevalonate
-
 ?
2.7.1.36 ATP + (R)-mevalonate
-
 Homo sapiens ADP + (R)-5-phosphomevalonate
-
 ?
2.7.1.36 ATP + (R)-mevalonate
-
 Rattus norvegicus ADP + (R)-5-phosphomevalonate
-
 ?
2.7.1.36 ATP + (R)-mevalonate involved in cholesterol synthesis Rattus norvegicus ADP + (R)-5-phosphomevalonate
-
 ?
2.7.1.36 ATP + (R)-mevalonate involved in cholesterol synthesis, decreased enzyme activity involved in mevalonic aciduria and hyperimmunoglobulin D syndrome Homo sapiens ADP + (R)-5-phosphomevalonate
-
 ?
2.7.1.36 ATP + (R)-mevalonate involved in isopentenyl diphosphate synthesis Staphylococcus aureus ADP + (R)-5-phosphomevalonate
-
 ?
2.7.1.36 ATP + mevalonate
-
 Staphylococcus aureus ADP + phosphomevalonate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.36 monomer 1 * 33000, SDS-PAGE and gel filtration Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.36 7 8.5
-
 Staphylococcus aureus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.36 6 9
-
 Staphylococcus aureus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.36 0.000029
-
 farnesyl thiodiphosphate pH 7.5, 30°C Homo sapiens
2.7.1.36 0.000035
-
 farnesyl diphosphate pH 7.5, 30°C Homo sapiens
2.7.1.36 0.00035
-
 farnesyl diphosphate pH 7.5, 30°C Rattus norvegicus
2.7.1.36 0.00047
-
 farnesyl thiodiphosphate pH 7.5, 30°C Rattus norvegicus
2.7.1.36 0.045
-
 farnesyl thiodiphosphate pH 7.5, 30°C Staphylococcus aureus
2.7.1.36 0.046
-
 farnesyl diphosphate pH 7.5, 30°C Staphylococcus aureus