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Literature summary extracted from

  • Rashid, N.; Kanai, T.; Atomi, H.; Imanaka, T.
    Among multiple phosphomannomutase gene orthologues, only one gene encodes a protein with phosphoglucomutase and phosphomannomutase activities in Thermococcus kodakaraensis (2004), J. Bacteriol., 186, 6070-6076.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.2.2 expression in Escherichia coli Thermococcus kodakarensis
5.4.2.8 expression in Escherichia coli Thermococcus kodakarensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.2.2 3
-
D-glucose-1-phosphate 90°C Thermococcus kodakarensis
5.4.2.2 3.2
-
D-mannose-1-phosphate 90°C Thermococcus kodakarensis
5.4.2.2 3.5
-
2-deoxyribose-1-phosphate 90°C Thermococcus kodakarensis
5.4.2.8 3
-
D-glucose-1-phosphate 90°C Thermococcus kodakarensis
5.4.2.8 3.2
-
D-mannose-1-phosphate 90°C Thermococcus kodakarensis
5.4.2.8 3.5
-
2-deoxyribose-1-phosphate 90°C Thermococcus kodakarensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.2.2 Mg2+
-
Thermococcus kodakarensis
5.4.2.2 additional information Ni2+, Mn2+ and Zn2+ at a concentration of 1 mM can also support activity, although to a lesser extent Thermococcus kodakarensis
5.4.2.8 Mg2+
-
Thermococcus kodakarensis
5.4.2.8 additional information Ni2+, Mn2+ and Zn2+ at a concentration of 1 mM can also support activity, although to a lesser extent Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.2.2 49850
-
4 * 49850, protein encoded by gene TK1108, calculated Thermococcus kodakarensis
5.4.2.2 210000
-
gel filtration Thermococcus kodakarensis
5.4.2.8 49850
-
4 * 49850, protein encoded by gene TK1108, calculated Thermococcus kodakarensis
5.4.2.8 210000
-
gel filtration Thermococcus kodakarensis

Organism

EC Number Organism UniProt Comment Textmining
5.4.2.2 Thermococcus kodakarensis
-
-
-
5.4.2.8 Thermococcus kodakarensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.2.2 heat treatment 85°C, 20 min, anion-exchange, hydrophobic and gel filtration column chromatography Thermococcus kodakarensis
5.4.2.8 heat treatment 85°C, 20 min, anion-exchange, hydrophobic and gel filtration column chromatography Thermococcus kodakarensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.2.2 additional information
-
2-deoxyribose-1-phosphate Vmax 0.230, 90°C Thermococcus kodakarensis
5.4.2.2 additional information
-
D-glucose-1-phosphate Vmax 0.690, 90°C, pH 7.0 Thermococcus kodakarensis
5.4.2.2 additional information
-
D-mannose-1-phosphate Vmax 0.401, 90°C Thermococcus kodakarensis
5.4.2.2 0.8
-
cell extract, pH 7.0 Thermococcus kodakarensis
5.4.2.2 420
-
purified enzyme, pH 7.0, 90°C Thermococcus kodakarensis
5.4.2.8 additional information
-
2-deoxyribose-1-phosphate Vmax 0.230, 90°C Thermococcus kodakarensis
5.4.2.8 additional information
-
D-glucose-1-phosphate Vmax 0.690, 90°C, pH 7.0 Thermococcus kodakarensis
5.4.2.8 additional information
-
D-mannose-1-phosphate Vmax 0.401, 90°C Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.2.2 2-deoxyribose 1-phosphate
-
Thermococcus kodakarensis 2-deoxyribose 5-phosphate
-
?
5.4.2.2 3-phospho-D-glyceric acid no mutase activity Thermococcus kodakarensis ?
-
?
5.4.2.2 D-fructose-1-phosphate no mutase activity Thermococcus kodakarensis D-fructose-6-phosphate
-
?
5.4.2.2 D-glucosamine-1-phosphate low enzyme activity Thermococcus kodakarensis D-glucosamine-6-phosphate
-
?
5.4.2.2 D-glucose-1-phosphate bifunctional enzyme Thermococcus kodakarensis D-glucose-6-phosphate
-
?
5.4.2.2 D-mannose-1-phosphate bifunctional enzyme Thermococcus kodakarensis D-mannose-6-phosphate
-
?
5.4.2.2 N-acetyl-D-glucosamine-1-phosphate no mutase activity Thermococcus kodakarensis N-acetyl-D-glucosamine-6-phosphate
-
?
5.4.2.8 2-deoxyribose 1-phosphate
-
Thermococcus kodakarensis 2-deoxyribose 5-phosphate
-
?
5.4.2.8 3-phospho-D-glyceric acid no mutase activity Thermococcus kodakarensis ?
-
?
5.4.2.8 D-fructose-1-phosphate no mutase activity Thermococcus kodakarensis D-fructose-6-phosphate
-
?
5.4.2.8 D-glucosamine-1-phosphate low enzyme activity Thermococcus kodakarensis D-glucosamine-6-phosphate
-
?
5.4.2.8 D-glucose-1-phosphate bifunctional enzyme Thermococcus kodakarensis D-glucose-6-phosphate
-
?
5.4.2.8 D-mannose-1-phosphate bifunctional enzyme Thermococcus kodakarensis D-mannose-6-phosphate
-
?
5.4.2.8 N-acetyl-D-glucosamine-1-phosphate no mutase activity Thermococcus kodakarensis N-acetyl-D-glucosamine-6-phosphate
-
?

Subunits

EC Number Subunits Comment Organism
5.4.2.2 tetramer 4 * 49850, protein encoded by gene TK1108, calculated Thermococcus kodakarensis
5.4.2.8 tetramer 4 * 49850, protein encoded by gene TK1108, calculated Thermococcus kodakarensis

Synonyms

EC Number Synonyms Comment Organism
5.4.2.2 PMM/PGM
-
Thermococcus kodakarensis
5.4.2.8 PMM/PGM
-
Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.2.2 90
-
-
Thermococcus kodakarensis
5.4.2.8 90
-
-
Thermococcus kodakarensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.4.2.2 100
-
highly stable, half-life of about 85 min in boiling water in presence of 10mM Mg2+ Thermococcus kodakarensis
5.4.2.8 100
-
highly stable, half-life of about 85 min in boiling water in presence of 10mM Mg2+ Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.2.2 7
-
10mM Mg2+, 90°C Thermococcus kodakarensis
5.4.2.8 7
-
10mM Mg2+, 90°C Thermococcus kodakarensis