EC Number | Cloned (Comment) | Organism |
---|---|---|
5.4.2.2 | expression in Escherichia coli | Thermococcus kodakarensis |
5.4.2.8 | expression in Escherichia coli | Thermococcus kodakarensis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.4.2.2 | 3 | - |
D-glucose-1-phosphate | 90°C | Thermococcus kodakarensis | |
5.4.2.2 | 3.2 | - |
D-mannose-1-phosphate | 90°C | Thermococcus kodakarensis | |
5.4.2.2 | 3.5 | - |
2-deoxyribose-1-phosphate | 90°C | Thermococcus kodakarensis | |
5.4.2.8 | 3 | - |
D-glucose-1-phosphate | 90°C | Thermococcus kodakarensis | |
5.4.2.8 | 3.2 | - |
D-mannose-1-phosphate | 90°C | Thermococcus kodakarensis | |
5.4.2.8 | 3.5 | - |
2-deoxyribose-1-phosphate | 90°C | Thermococcus kodakarensis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.4.2.2 | Mg2+ | - |
Thermococcus kodakarensis | |
5.4.2.2 | additional information | Ni2+, Mn2+ and Zn2+ at a concentration of 1 mM can also support activity, although to a lesser extent | Thermococcus kodakarensis | |
5.4.2.8 | Mg2+ | - |
Thermococcus kodakarensis | |
5.4.2.8 | additional information | Ni2+, Mn2+ and Zn2+ at a concentration of 1 mM can also support activity, although to a lesser extent | Thermococcus kodakarensis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.4.2.2 | 49850 | - |
4 * 49850, protein encoded by gene TK1108, calculated | Thermococcus kodakarensis |
5.4.2.2 | 210000 | - |
gel filtration | Thermococcus kodakarensis |
5.4.2.8 | 49850 | - |
4 * 49850, protein encoded by gene TK1108, calculated | Thermococcus kodakarensis |
5.4.2.8 | 210000 | - |
gel filtration | Thermococcus kodakarensis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.4.2.2 | Thermococcus kodakarensis | - |
- |
- |
5.4.2.8 | Thermococcus kodakarensis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.4.2.2 | heat treatment 85°C, 20 min, anion-exchange, hydrophobic and gel filtration column chromatography | Thermococcus kodakarensis |
5.4.2.8 | heat treatment 85°C, 20 min, anion-exchange, hydrophobic and gel filtration column chromatography | Thermococcus kodakarensis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.4.2.2 | additional information | - |
2-deoxyribose-1-phosphate Vmax 0.230, 90°C | Thermococcus kodakarensis |
5.4.2.2 | additional information | - |
D-glucose-1-phosphate Vmax 0.690, 90°C, pH 7.0 | Thermococcus kodakarensis |
5.4.2.2 | additional information | - |
D-mannose-1-phosphate Vmax 0.401, 90°C | Thermococcus kodakarensis |
5.4.2.2 | 0.8 | - |
cell extract, pH 7.0 | Thermococcus kodakarensis |
5.4.2.2 | 420 | - |
purified enzyme, pH 7.0, 90°C | Thermococcus kodakarensis |
5.4.2.8 | additional information | - |
2-deoxyribose-1-phosphate Vmax 0.230, 90°C | Thermococcus kodakarensis |
5.4.2.8 | additional information | - |
D-glucose-1-phosphate Vmax 0.690, 90°C, pH 7.0 | Thermococcus kodakarensis |
5.4.2.8 | additional information | - |
D-mannose-1-phosphate Vmax 0.401, 90°C | Thermococcus kodakarensis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.2.2 | 2-deoxyribose 1-phosphate | - |
Thermococcus kodakarensis | 2-deoxyribose 5-phosphate | - |
? | |
5.4.2.2 | 3-phospho-D-glyceric acid | no mutase activity | Thermococcus kodakarensis | ? | - |
? | |
5.4.2.2 | D-fructose-1-phosphate | no mutase activity | Thermococcus kodakarensis | D-fructose-6-phosphate | - |
? | |
5.4.2.2 | D-glucosamine-1-phosphate | low enzyme activity | Thermococcus kodakarensis | D-glucosamine-6-phosphate | - |
? | |
5.4.2.2 | D-glucose-1-phosphate | bifunctional enzyme | Thermococcus kodakarensis | D-glucose-6-phosphate | - |
? | |
5.4.2.2 | D-mannose-1-phosphate | bifunctional enzyme | Thermococcus kodakarensis | D-mannose-6-phosphate | - |
? | |
5.4.2.2 | N-acetyl-D-glucosamine-1-phosphate | no mutase activity | Thermococcus kodakarensis | N-acetyl-D-glucosamine-6-phosphate | - |
? | |
5.4.2.8 | 2-deoxyribose 1-phosphate | - |
Thermococcus kodakarensis | 2-deoxyribose 5-phosphate | - |
? | |
5.4.2.8 | 3-phospho-D-glyceric acid | no mutase activity | Thermococcus kodakarensis | ? | - |
? | |
5.4.2.8 | D-fructose-1-phosphate | no mutase activity | Thermococcus kodakarensis | D-fructose-6-phosphate | - |
? | |
5.4.2.8 | D-glucosamine-1-phosphate | low enzyme activity | Thermococcus kodakarensis | D-glucosamine-6-phosphate | - |
? | |
5.4.2.8 | D-glucose-1-phosphate | bifunctional enzyme | Thermococcus kodakarensis | D-glucose-6-phosphate | - |
? | |
5.4.2.8 | D-mannose-1-phosphate | bifunctional enzyme | Thermococcus kodakarensis | D-mannose-6-phosphate | - |
? | |
5.4.2.8 | N-acetyl-D-glucosamine-1-phosphate | no mutase activity | Thermococcus kodakarensis | N-acetyl-D-glucosamine-6-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.4.2.2 | tetramer | 4 * 49850, protein encoded by gene TK1108, calculated | Thermococcus kodakarensis |
5.4.2.8 | tetramer | 4 * 49850, protein encoded by gene TK1108, calculated | Thermococcus kodakarensis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.4.2.2 | PMM/PGM | - |
Thermococcus kodakarensis |
5.4.2.8 | PMM/PGM | - |
Thermococcus kodakarensis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.4.2.2 | 90 | - |
- |
Thermococcus kodakarensis |
5.4.2.8 | 90 | - |
- |
Thermococcus kodakarensis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.4.2.2 | 100 | - |
highly stable, half-life of about 85 min in boiling water in presence of 10mM Mg2+ | Thermococcus kodakarensis |
5.4.2.8 | 100 | - |
highly stable, half-life of about 85 min in boiling water in presence of 10mM Mg2+ | Thermococcus kodakarensis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.4.2.2 | 7 | - |
10mM Mg2+, 90°C | Thermococcus kodakarensis |
5.4.2.8 | 7 | - |
10mM Mg2+, 90°C | Thermococcus kodakarensis |