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Literature summary extracted from

  • Kanamori, T.; Kanou, N.; Atomi, H.; Imanaka, T.
    Enzymatic characterization of a prokaryotic urea carboxylase (2004), J. Bacteriol., 186, 2532-2539.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.6 expression in Escherichia coli Oleomonas sagaranensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.6 7.17
-
 Urea 30°C, pH 7.5 Oleomonas sagaranensis
6.3.4.6 56.5
-
 Formamide 30°C, pH 7.5 Oleomonas sagaranensis
6.3.4.6 126
-
 acetamide 30°C, pH 7.5 Oleomonas sagaranensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.6 Co2+ divalent cation required, Mg2+, Mn2+ or Co2+ support activity Oleomonas sagaranensis
6.3.4.6 Mg2+ divalent cation is required for activity. Increase in activity at concentrations up to 4 mM Mg2+, higher concentrations do not inhibit Oleomonas sagaranensis
6.3.4.6 Mn2+ divalent cation required, Mg2+, Mn2+ or Co2+ support activity Oleomonas sagaranensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.6 ATP + urea + CO2 Oleomonas sagaranensis activity is inducible by urea, acetamide or formamide ADP + phosphate + urea-1-carboxylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.6 Oleomonas sagaranensis Q75RY4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.6 recombinant Oleomonas sagaranensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.6 additional information
-
-
 Oleomonas sagaranensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.6 ATP + acetamide + HCO3-
-
 Oleomonas sagaranensis ?
-
 ?
6.3.4.6 ATP + formamide + HCO3-
-
 Oleomonas sagaranensis ?
-
 ?
6.3.4.6 ATP + urea + CO2 activity is inducible by urea, acetamide or formamide Oleomonas sagaranensis ADP + phosphate + urea-1-carboxylate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.4.6 UCA
-
 Oleomonas sagaranensis
6.3.4.6 urea carboxylase
-
 Oleomonas sagaranensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.4.6 16.8
-
 Formamide 30°C, pH 7.5 Oleomonas sagaranensis
6.3.4.6 40.4
-
 acetamide 30°C, pH 7.5 Oleomonas sagaranensis
6.3.4.6 44.2
-
 Urea 30°C, pH 7.5 Oleomonas sagaranensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.6 7 7.5 HEPES-NaOH buffer Oleomonas sagaranensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.4.6 6.5 8.5 pH 6.5: about 80% of maximal activity, pH 8.5: about 45% of maximal activity, Hepes-NaOH buffer Oleomonas sagaranensis