Literature summary extracted from
Furukawa, K.; Arimura, N.
Purification and properties of 2,3-dihydroxybiphenyl dioxygenase from polychlorinated biphenyl-degrading Pseudomonas pseudoalcaligenes and Pseudomonas aeruginosa carrying the cloned bphC gene (1987), J. Bacteriol., 169, 924-927.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.13.11.39 |
expressed in Pseudomonas aeruginosa |
Pseudomonas oleovorans |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.13.11.39 |
Fe3+ |
complete inactivation |
Pseudomonas oleovorans |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
1.13.11.39 |
0.087 |
- |
biphenyl-2,3-diol |
pH 9.0, 25°C |
Pseudomonas oleovorans |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.13.11.39 |
Fe2+ |
required, after loss of ferrous iron, activity can be restored by incubation with ferrous iron in presence of cysteine |
Pseudomonas oleovorans |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.13.11.39 |
31000 |
- |
8 * 31000, SDS-PAGE |
Pseudomonas oleovorans |
1.13.11.39 |
250000 |
- |
PAGE |
Pseudomonas oleovorans |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.13.11.39 |
Pseudomonas oleovorans |
- |
native enzyme and expressed in Pseudomonas aeruginosa |
- |
Renatured (Commentary)
EC Number |
Renatured (Comment) |
Organism |
---|
1.13.11.39 |
after loss of ferrous iron, activity can be restored by incubation with ferrous iron in presence of cysteine |
Pseudomonas oleovorans |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
1.13.11.39 |
87.2 |
- |
pH 9.0, 25°C |
Pseudomonas oleovorans |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
1.13.11.39 |
stable after addition of 10% acetone or 10% ethanol |
Pseudomonas oleovorans |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.13.11.39 |
biphenyl-2,3-diol + O2 |
- |
Pseudomonas oleovorans |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O |
- |
? |
|
1.13.11.39 |
additional information |
no substrate: biphenyl-3,4-diol, catechol, 3-methylcatechol, 4-methylcatechol |
Pseudomonas oleovorans |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.13.11.39 |
More |
N-terminal amino acid sequence |
Pseudomonas oleovorans |
1.13.11.39 |
octamer |
8 * 31000, SDS-PAGE |
Pseudomonas oleovorans |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.13.11.39 |
9 |
- |
- |
Pseudomonas oleovorans |
pI Value
EC Number |
Organism |
Comment |
pI Value Maximum |
pI Value |
---|
1.13.11.39 |
Pseudomonas oleovorans |
isoelectric focusing |
- |
4.5 |