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Literature summary extracted from

  • Furukawa, K.; Arimura, N.
    Purification and properties of 2,3-dihydroxybiphenyl dioxygenase from polychlorinated biphenyl-degrading Pseudomonas pseudoalcaligenes and Pseudomonas aeruginosa carrying the cloned bphC gene (1987), J. Bacteriol., 169, 924-927.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.39 expressed in Pseudomonas aeruginosa Pseudomonas oleovorans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.39 Fe3+ complete inactivation Pseudomonas oleovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.39 0.087
-
biphenyl-2,3-diol pH 9.0, 25°C Pseudomonas oleovorans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.39 Fe2+ required, after loss of ferrous iron, activity can be restored by incubation with ferrous iron in presence of cysteine Pseudomonas oleovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.39 31000
-
8 * 31000, SDS-PAGE Pseudomonas oleovorans
1.13.11.39 250000
-
PAGE Pseudomonas oleovorans

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.39 Pseudomonas oleovorans
-
native enzyme and expressed in Pseudomonas aeruginosa
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.13.11.39 after loss of ferrous iron, activity can be restored by incubation with ferrous iron in presence of cysteine Pseudomonas oleovorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.39 87.2
-
pH 9.0, 25°C Pseudomonas oleovorans

Storage Stability

EC Number Storage Stability Organism
1.13.11.39 stable after addition of 10% acetone or 10% ethanol Pseudomonas oleovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.39 biphenyl-2,3-diol + O2
-
Pseudomonas oleovorans 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
?
1.13.11.39 additional information no substrate: biphenyl-3,4-diol, catechol, 3-methylcatechol, 4-methylcatechol Pseudomonas oleovorans ?
-
?

Subunits

EC Number Subunits Comment Organism
1.13.11.39 More N-terminal amino acid sequence Pseudomonas oleovorans
1.13.11.39 octamer 8 * 31000, SDS-PAGE Pseudomonas oleovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.39 9
-
-
Pseudomonas oleovorans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.13.11.39 Pseudomonas oleovorans isoelectric focusing
-
4.5