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Literature summary extracted from
- Enzyme redesign: two mutations cooperate to convert cycloartenol synthase into an accurate lanosterol synthase (2005), J. Am. Chem. Soc., 127, 14132-14133.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.8 | expression in Saccharomyces cerevisiae | Arabidopsis thaliana |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.4.99.8 | H477N | produces 88% lanosterol and 12% parkeol | Arabidopsis thaliana |
| 5.4.99.8 | H477N/I481V | produces 99% lanosterol and 1% parkeol | Arabidopsis thaliana |
| 5.4.99.8 | H477Q | produces 22% lanosterol, 73% parkeol, and 5% 9-beta-delta-7-lanosterol | Arabidopsis thaliana |
| 5.4.99.8 | H477Q/I481V | produces 94% lanosterol and 6% parkeol | Arabidopsis thaliana |
| 5.4.99.8 | I481V | produces 54% cycloartenol, 25% lanosterol, and 21% parkeol | Arabidopsis thaliana |
| 5.4.99.8 | Y410T | produces 65% lanosterol, 2% parkeol, and 33% 9-beta-delta-7-lanosterol | Arabidopsis thaliana |
| 5.4.99.8 | Y410T/H477N/I481V | produces 78% lanosterol and 22% 9-beta-delta-7-lanosterol | Arabidopsis thaliana |
| 5.4.99.8 | Y410T/H477Q/I481V | produces 78% lanosterol and 22% 9-beta-delta-7-lanosterol | Arabidopsis thaliana |
| 5.4.99.8 | Y410T/I481V | produces 78% lanosterol, less than 1% parkeol, and 22% 9-beta-delta-7-lanosterol | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.8 | (S)-2,3-Epoxysqualene | Arabidopsis thaliana | - |
Cycloartenol | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.8 | Arabidopsis thaliana | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.8 | - |
Arabidopsis thaliana |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.8 | (S)-2,3-Epoxysqualene | - |
Arabidopsis thaliana | Cycloartenol | - |
? | |
| 5.4.99.8 | (S)-2,3-oxidosqualene | - |
Arabidopsis thaliana | cycloartenol | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.8 | cycloartenol synthase | - |
Arabidopsis thaliana |
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Release 2023.1 (January 2023)
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