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Literature summary extracted from

  • Pilla, C.; Cardozo, R.F.; Dornelles, P.K.; Dutra-Filho, C.S.; Wyse, A.T.; Wajner, M.; Wannmacher, C.M.
    Kinetic studies on the inhibition of creatine kinase activity by branched-chain alpha-amino acids in the brain cortex of rats (2003), Int. J. Dev. Neurosci., 21, 145-151.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.3.2 4-hydroxymercuribenzoic acid
-
 Rattus norvegicus
2.7.3.2 L-isoleucine branched chain alpha-amino acids bind at the active site, competitive inhibition mechanism against substrates phosphocreatine and ADP, inhibition kinetics Rattus norvegicus
2.7.3.2 L-leucine branched chain alpha-amino acids bind at the active site, competitive inhibition mechanism against substrates phosphocreatine and ADP, inhibition kinetics Rattus norvegicus
2.7.3.2 L-valine branched chain alpha-amino acids bind at the active site, competitive inhibition mechanism against substrates phosphocreatine and ADP, inhibition kinetics Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.3.2 additional information
-
 additional information kinetics Rattus norvegicus
2.7.3.2 0.3
-
 ADP pH 7.5, 37°C Rattus norvegicus
2.7.3.2 0.8
-
 phosphocreatine pH 7.5, 37°C Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.3.2 Mg2+
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.3.2 ATP + creatine Rattus norvegicus key enzyme of energy homeostasis ADP + phosphocreatine
-
 r
2.7.3.2 additional information Rattus norvegicus enzyme inhibition, e.g. by branched chain alpha-amino acids, might contribute to the brain damage maple syrup urine disease MSUD ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.2 Rattus norvegicus
-
 21-day-old Wistar rats
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.3.2 brain cortex
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.2 ATP + creatine
-
 Rattus norvegicus ADP + phosphocreatine
-
 r
2.7.3.2 ATP + creatine key enzyme of energy homeostasis Rattus norvegicus ADP + phosphocreatine
-
 r
2.7.3.2 additional information enzyme inhibition, e.g. by branched chain alpha-amino acids, might contribute to the brain damage maple syrup urine disease MSUD Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.3.2 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.3.2 7.5
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.3.2 ADP
-
 Rattus norvegicus
2.7.3.2 ATP
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.3.2 10
-
 L-isoleucine versus phosphocreatine, pH 7.5, 37°C Rattus norvegicus
2.7.3.2 14
-
 L-valine versus phosphocreatine, pH 7.5, 37°C Rattus norvegicus
2.7.3.2 16
-
 L-leucine versus phosphocreatine, pH 7.5, 37°C Rattus norvegicus
2.7.3.2 21
-
 L-isoleucine versus ADP, pH 7.5, 37°C Rattus norvegicus
2.7.3.2 22
-
 L-leucine versus ADP, pH 7.5, 37°C Rattus norvegicus
2.7.3.2 23
-
 L-valine versus ADP, pH 7.5, 37°C Rattus norvegicus