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Literature summary extracted from

  • Uda, K.; Tanaka, K.; Bailly, X.; Zal, F.; Suzuki, T.
    Phosphagen kinase of the giant tubeworm Riftia pachyptila. Cloning and expression of cytoplasmic and mitochondrial isoforms of taurocyamine kinase (2005), Int. J. Biol. Macromol., 37, 54-60.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.3.4 expression in Escherichia coli TB1 Riftia pachyptila

General Stability

EC Number General Stability Organism
2.7.3.4 structurally very unstable, gradual loss of enzyme activity even when it is stored on ice for several hours Riftia pachyptila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.3.4 0.881
-
 Taurocyamine
-
 Arenicola brasiliensis
2.7.3.4 2.12
-
 Taurocyamine
-
 Riftia pachyptila
2.7.3.4 4.01
-
 Taurocyamine
-
 Arenicola brasiliensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.3.4 cytoplasm
-
 Riftia pachyptila 5737
-
2.7.3.4 cytoplasm
-
 Arenicola brasiliensis 5737
-
2.7.3.4 mitochondrion
-
 Riftia pachyptila 5739
-
2.7.3.4 mitochondrion
-
 Arenicola brasiliensis 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.3.4 40830
-
 calculated from amino acid sequence Riftia pachyptila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.3.4 ATP + taurocyamine Riftia pachyptila key role in the interconnection between energy production and utilization in animals ADP + N-phosphotaurocyamine
-
 ?
2.7.3.4 ATP + taurocyamine Arenicola brasiliensis key role in the interconnection between energy production and utilization in animals ADP + N-phosphotaurocyamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.4 Arenicola brasiliensis
-
-
-
2.7.3.4 Riftia pachyptila Q4AEC7
-
-
2.7.3.4 Riftia pachyptila Q4AEC8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.3.4 recombinant protein is purified by affinity chromatography using amylose resin Riftia pachyptila

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.3.4 plume a small part of the plume Riftia pachyptila
-
2.7.3.4 vestimentum
-
 Riftia pachyptila
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.3.4 0.298
-
 arginine Riftia pachyptila
2.7.3.4 0.855
-
 lombricine Riftia pachyptila
2.7.3.4 3.25
-
 lombricine Riftia pachyptila
2.7.3.4 3.65
-
 glycocyamine Riftia pachyptila
2.7.3.4 4.04
-
 taurocyamine Riftia pachyptila
2.7.3.4 10.4
-
 taurocyamine Riftia pachyptila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.4 ATP + arginine 2.9% of taurocyamine activity Riftia pachyptila ADP + ?
-
 ?
2.7.3.4 ATP + glycocyamine no activity Riftia pachyptila ADP + N-phosphoglycocyamine
-
 ?
2.7.3.4 ATP + glycocyamine 35% of taurocyamine activity Riftia pachyptila ADP + N-phosphoglycocyamine
-
 ?
2.7.3.4 ATP + lombricine 21% of taurocyamine activity Riftia pachyptila ADP + N-phospholombricine
-
 ?
2.7.3.4 ATP + lombricine 31% of taurocyamine activity Riftia pachyptila ADP + N-phospholombricine
-
 ?
2.7.3.4 ATP + taurocyamine
-
 Riftia pachyptila ADP + N-phosphotaurocyamine
-
 ?
2.7.3.4 ATP + taurocyamine
-
 Arenicola brasiliensis ADP + N-phosphotaurocyamine
-
 ?
2.7.3.4 ATP + taurocyamine key role in the interconnection between energy production and utilization in animals Riftia pachyptila ADP + N-phosphotaurocyamine
-
 ?
2.7.3.4 ATP + taurocyamine key role in the interconnection between energy production and utilization in animals Arenicola brasiliensis ADP + N-phosphotaurocyamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.3.4 MiTK
-
 Arenicola brasiliensis
2.7.3.4 phosphagen kinase
-
 Riftia pachyptila
2.7.3.4 taurocyamine kinase
-
 Riftia pachyptila
2.7.3.4 taurocyamine kinase
-
 Arenicola brasiliensis
2.7.3.4 TK
-
 Arenicola brasiliensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.3.4 9.43
-
 Taurocyamine
-
 Arenicola brasiliensis
2.7.3.4 12.5
-
 Taurocyamine
-
 Riftia pachyptila
2.7.3.4 14.3
-
 Taurocyamine
-
 Arenicola brasiliensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.3.4 Riftia pachyptila calculated
-
 7.98