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Literature summary extracted from

  • Wongtrakul, J.; Sramala, I.; Prapanthadara, L.A.; Ketterman, A.J.
    Intra-subunit residue interactions from the protein surface to the active site of glutathione S-transferase AdGSTD3-3 impact on structure and enzyme properties (2005), Insect Biochem. Mol. Biol., 35, 197-205.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.18 D150A turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 1.3fold higher than wild-type value. KM-value for 1-chloro-2,4-dinitrobenzene is 2fold higher than wild-type value Anopheles dirus
2.5.1.18 D150S turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 1.1fold higher than wild-type value. KM-value for 1-chloro-2,4-dinitrobenzene is 2fold higher than wild-type value Anopheles dirus
2.5.1.18 D150Y turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 1.2fold higher than wild-type value. KM-value for 1-chloro-2,4-dinitrobenzene is 1.7fold higher than wild-type value Anopheles dirus
2.5.1.18 H144A turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 1.2fold higher than wild-type value. KM-value for 1-chloro-2,4-dinitrobenzene is nearly identical to wild-type value Anopheles dirus
2.5.1.18 R96A turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 1.9fold higher than wild-type value. KM-value for 1-chloro-2,4-dinitrobenzene is 1.5fold higher than wild-type value Anopheles dirus
2.5.1.18 V147A turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene nearly identical to wild-type value. KM-value for 1-chloro-2,4-dinitrobenzene is 1.5fold lower than wild-type value Anopheles dirus
2.5.1.18 V147L turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 1.2fold lower than wild-type value. KM-value for 1-chloro-2,4-dinitrobenzene is 1.8fold lower than wild-type value Anopheles dirus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 1,2-dichloro-4-nitrobenzene 1 mM, 53.3% inhibition of wild-type enzyme, 40.4% of mutant enzyme H144A, 42.4% of mutant enzyme V147A, 54.3% of mutant enzyme V147L, 35.9% of mutant enzyme R96A Anopheles dirus
2.5.1.18 4-nitrobenzyl chloride 1 mM, 50.5% inhibition of wild-type enzyme, 43.9% of mutant enzyme H144A, 40.8% of mutant enzyme V147A, 47.9% of mutant enzyme V147L, 30.8% of mutant enzyme R96A Anopheles dirus
2.5.1.18 4-nitrophenethyl bromide 0.1 mM, 40.1% inhibition of wild-type enzyme, 23.6% of mutant enzyme H144A, 19.5% of mutant enzyme V147A, 31.4% of mutant enzyme V147L, 27.3% of mutant enzyme R96A Anopheles dirus
2.5.1.18 cumene hydroperoxide 2.5 mM, 43.3% inhibition of wild-type enzyme, 27% of mutant enzyme H144A, 50.6% of mutant enzyme V147A, 41.5% of mutant enzyme V147L, 48.1% of mutant enzyme R96A Anopheles dirus
2.5.1.18 deltamethrin 0.01 mM, 65.4% inhibition of wild-type enzyme, 44.2% of mutant enzyme H144A, 59.5% of mutant enzyme V147A, 66.1% of mutant enzyme V147L, 88.6% of mutant enzyme R96A Anopheles dirus
2.5.1.18 Ethacrynic acid 0.001 mM, 35.3% inhibition of wild-type enzyme, 32.7% of mutant enzyme H144A, 27.8% of mutant enzyme V147A, 23.0% of mutant enzyme V147L, 29.1% of mutant enzyme R96A Anopheles dirus
2.5.1.18 permethrin 0.01 mM, 51.1% inhibition of wild-type enzyme, 38.8% of mutant enzyme H144A, 53.1% of mutant enzyme V147A, 49% of mutant enzyme V147L, 65.5% of mutant enzyme R96A Anopheles dirus
2.5.1.18 S-hexyl glutathione 0.01 mM, 21.7% inhibition of wild-type enzyme, 23% of mutant enzyme H144A, 10.8% of mutant enzyme V147A, 24.2% of mutant enzyme V147L, 14.7% of mutant enzyme R96A Anopheles dirus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.07
-
 1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, mutant enzyme D150S Anopheles dirus
2.5.1.18 0.08
-
 1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, mutant enzyme D150A Anopheles dirus
2.5.1.18 0.09
-
 1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, mutant enzyme D150Y Anopheles dirus
2.5.1.18 0.13
-
 1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, wild-type enzyme Anopheles dirus
2.5.1.18 0.14
-
 1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, mutant enzyme H144A Anopheles dirus
2.5.1.18 0.17
-
 reduced glutathione 25-27°C, pH 6.5, mutant enzyme D150S Anopheles dirus
2.5.1.18 0.19
-
 1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, mutant enzyme V147A Anopheles dirus
2.5.1.18 0.2
-
 1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, mutant enzyme V147L Anopheles dirus
2.5.1.18 0.21
-
 reduced glutathione 25-27°C, pH 6.5, mutant enzyme D150Y Anopheles dirus
2.5.1.18 0.26
-
 reduced glutathione 25-27°C, pH 6.5, mutant enzyme V147A Anopheles dirus
2.5.1.18 0.29
-
 reduced glutathione 25-27°C, pH 6.5, wild-type enzyme Anopheles dirus
2.5.1.18 0.33
-
 reduced glutathione 25-27°C, pH 6.5, mutant enzyme D150A Anopheles dirus
2.5.1.18 0.34
-
 reduced glutathione 25-27°C, pH 6.5, mutant enzyme V147L Anopheles dirus
2.5.1.18 0.37
-
 reduced glutathione 25-27°C, pH 6.5, mutant enzyme H144A Anopheles dirus
2.5.1.18 0.38
-
 1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, mutant enzyme R96A Anopheles dirus
2.5.1.18 2.75
-
 reduced glutathione 25-27°C, pH 6.5, mutant enzyme R96A Anopheles dirus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Anopheles dirus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 reduced glutathione + 1-chloro-2,4-dinitrobenzene
-
 Anopheles dirus S-2,4-dinitrophenylglutathione + HCl
-
 ?
2.5.1.18 reduced glutathione + 4-nitrobenzyl chloride
-
 Anopheles dirus ?
-
 ?
2.5.1.18 reduced glutathione + 4-nitrophenethyl bromide
-
 Anopheles dirus ?
-
 ?
2.5.1.18 reduced glutathione + ethacrynic acid
-
 Anopheles dirus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 glutathione S-transferase AdFSTD3-3
-
 Anopheles dirus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.18 21.6
-
 1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, mutant enzyme V147L Anopheles dirus
2.5.1.18 21.6
-
 reduced glutathione 25-27°C, pH 6.5, mutant enzyme V147L Anopheles dirus
2.5.1.18 24.9
-
 1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, mutant enzyme V147A Anopheles dirus
2.5.1.18 24.9
-
 reduced glutathione 25-27°C, pH 6.5, mutant enzyme V147A Anopheles dirus
2.5.1.18 25.2
-
 1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, wild-type enzyme Anopheles dirus
2.5.1.18 25.2
-
 reduced glutathione 25-27°C, pH 6.5, wild-type enzyme Anopheles dirus
2.5.1.18 27.8
-
 1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, mutant enzyme D150S Anopheles dirus
2.5.1.18 27.8
-
 reduced glutathione 25-27°C, pH 6.5, mutant enzyme D150S Anopheles dirus
2.5.1.18 29.1
-
 1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, mutant enzyme H144A Anopheles dirus
2.5.1.18 29.1
-
 reduced glutathione 25-27°C, pH 6.5, mutant enzyme H144A Anopheles dirus
2.5.1.18 29.5
-
 1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, mutant enzyme D150Y Anopheles dirus
2.5.1.18 29.5
-
 reduced glutathione 25-27°C, pH 6.5, mutant enzyme D150Y Anopheles dirus
2.5.1.18 31.7
-
 1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, mutant enzyme D150A Anopheles dirus
2.5.1.18 31.7
-
 reduced glutathione 25-27°C, pH 6.5, mutant enzyme D150A Anopheles dirus
2.5.1.18 46.8
-
 1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, mutant enzyme R96A Anopheles dirus
2.5.1.18 46.8
-
 reduced glutathione 25-27°C, pH 6.5, mutant enzyme R96A Anopheles dirus