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Literature summary extracted from

  • Patel, R.; Soulages, J.L.; Wells, M.A.; Arrese, E.L.
    cAMP-dependent protein kinase of Manduca sexta phosphorylates but does not activate the fat body triglyceride lipase (2004), Insect Biochem. Mol. Biol., 34, 1269-1279.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.11 adipokinetic hormone rapid enzyme activation Manduca sexta
2.7.11.11 cAMP dependent on, 450% activation Manduca sexta

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.11 Ca2+ strong inhibition Manduca sexta
2.7.11.11 ethylmaleimide complete inhibition at 2 mM Manduca sexta
2.7.11.11 genistein partial inhibition Manduca sexta
2.7.11.11 H-89 potent inhibitor of PKA Manduca sexta
2.7.11.11 HA1077 partial inhibition Manduca sexta
2.7.11.11 Mn2+ strong inhibition Manduca sexta
2.7.11.11 PKI specific PKA inhibitor protein Manduca sexta
2.7.11.11 staurosporine partial inhibition Manduca sexta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.11 0.031
-
Kemptide pH 7.0, 22°C, catalytic subunit Manduca sexta
2.7.11.11 0.039
-
ATP pH 7.0, 22°C, catalytic subunit Manduca sexta
2.7.11.11 0.039
-
ATP ATP in form of MgATP2- Manduca sexta
2.7.11.11 0.73
-
histone pH 7.0, 22°C, catalytic subunit Manduca sexta

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.11 cytosol
-
Manduca sexta 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.11 Mg2+ absolutely required, as MgATP2-, best at 0.5 mM Mg2+, inhibitory at higher Mg2+ concentrations, cannot be substituted by Mn2+ Manduca sexta

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.11 45100
-
1 * 45100, isolated catalytic subunit, SDS-PAGE Manduca sexta

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.11 additional information Manduca sexta the enzyme plays a central role in the adipokinetic signaling controling the mobilization of stored lipids in the fat body ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.11 Manduca sexta
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.11 enzyme catalytic subunit from fat body 1773fold to homogeneity by ultracentrifugation and 3 steps of ion exchange chromatography Manduca sexta

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.11 fat body
-
Manduca sexta
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.11 0.266
-
purified catalytic subunit Manduca sexta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.11 ATP + fat body triglyceride lipase protein substrate is phosphorylated at Ser563, but not activated by the enzyme, Ser563 is the regulatory site of the enzyme substrate Manduca sexta ADP + phosphorylated fat body triglyceride lipase
-
?
2.7.11.11 ATP + histone
-
Manduca sexta ADP + phosphorylated histone
-
?
2.7.11.11 ATP + Kemptide
-
Manduca sexta ADP + phosphorylated Kemptide
-
?
2.7.11.11 ATP + LRRASLG i.e. Kemptide Manduca sexta ADP + LRRA-phosphoserine-LG
-
?
2.7.11.11 ATP + PLARTLSVAGLPGKK syntide 2-derived peptide substrate Manduca sexta ADP + PLARTL-phosphoserine-VAGLPGKK
-
?
2.7.11.11 ATP + RFARKGSLRQKNV PKC-derived peptide substrate Manduca sexta ADP + RFARKG-phosphoserine-LRQKNV
-
?
2.7.11.11 additional information the enzyme plays a central role in the adipokinetic signaling controling the mobilization of stored lipids in the fat body Manduca sexta ?
-
?
2.7.11.11 additional information substrate specificity, no activity with peptides derived from Thr-kinase or Tyr-kinase, overview Manduca sexta ?
-
?

Subunits

EC Number Subunits Comment Organism
2.7.11.11 monomer 1 * 45100, isolated catalytic subunit, SDS-PAGE Manduca sexta
2.7.11.11 More 2 catalytic subunits and 2 regulatory subunits form a tetramer, the inactive holoenzyme, upon cAMP binding the catalytic subunits are released as monomers and become catalytically active Manduca sexta

Synonyms

EC Number Synonyms Comment Organism
2.7.11.11 PKA
-
Manduca sexta

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.11 22
-
assay at room temperature Manduca sexta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.11 7
-
-
Manduca sexta

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.11 ATP as MgATP2- Manduca sexta

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.11.11 0.000048
-
H-89 pH 7.0, 22°C, catalytic subunit Manduca sexta

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.11.11 Manduca sexta large differences between catalytic and regulatory subunits
-
additional information